Development of anonymous cDNA microarrays to study changes to the Senecio floral transcriptome during hybrid speciation
Development of anonymous cDNA microarrays to study changes to the Senecio floral transcriptome during hybrid speciation
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DOI：
10.1111/j.1365-294x.2005.02608.x
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发表时间：
2005-07-01
影响因子：
4.9
通讯作者：
Hiscock, SJ
中科院分区：
文献类型：
--
作者：
Hegarty, MJ;Jones, JM;Hiscock, SJ
种间杂交是发生突然物种形成的重要过程。近年来，利用AFLP/SSR、GISH/FISH和cDNA-AFLP差异显示等技术对杂种形成过程中的遗传变化进行了研究。然而，在使用微阵列技术来分析与杂交物种形成相关的全基因组/转录组变化的进展一直是有限的，由于缺乏广泛的序列数据，许多杂交物种和外推的结果从市售的微阵列模型物种nonmodel杂交类群的困难。因此，越来越多的研究人员正在研究非模型系统转向“匿名”cDNA微阵列的发展，其中通过打印未测序的cDNA克隆和仅测序那些显示有趣的表达模式的克隆来减少生产微阵列的时间和成本。在这里，我们描述了创建，测试和初步使用的匿名cDNA微阵列研究花转录组的变化与异源多倍体物种形成千里光属。我们报告的异源六倍体杂交，千里光，其父母类群千里光squalidus（二倍体）和千里光（四倍体），和中间三倍体（不育）的杂交千里光之间的基因表达的比较。匿名微阵列分析揭示了这四个类群之间的花基因表达的显着差异，并证明了这种技术的力量，在nonmodel开花植物杂交的遗传影响的研究。
Interspecific hybridization is an important process through which abrupt speciation can occur. In recent years, genetic changes associated with hybrid speciation have been identified through a variety of techniques, including AFLP/SSR mapping, GISH/FISH and cDNA-AFLP differential display. However, progress in using microarray technology to analyse whole genome/transcriptome changes associated with hybrid speciation has been limited due to the lack of extensive sequence data for many hybrid species and the difficulties in extrapolating results from commercially available microarrays for model species onto nonmodel hybrid taxa. Increasingly therefore researchers studying nonmodel systems are turning to the development of 'anonymous' cDNA microarrays, where the time and cost of producing microarrays is reduced by printing unsequenced cDNA clones, and sequencing only those clones that display interesting expression patterns. Here we describe the creation, testing and preliminary use of anonymous cDNA microarrays to study changes in floral transcriptome associated with allopolyploid speciation in the genus Senecio. We report a comparison of gene expression between the allohexaploid hybrid, Senecio cambrensis, its parental taxa Senecio squalidus (diploid) and Senecio vulgaris (tetraploid), and the intermediate triploid (sterile) hybrid Senecioxbaxteri. Anonymous microarray analysis revealed dramatic differences in floral gene expression between these four taxa and demonstrates the power of this technique for studies of the genetic impact of hybridization in nonmodel flowering plants.