Glutathione Reduction of Patulin-Evoked Cytotoxicity in HEK293 Cells by the Prevention of Oxidative Damage and the Mitochondrial Apoptotic Pathway
Glutathione Reduction of Patulin-Evoked Cytotoxicity in HEK293 Cells by the Prevention of Oxidative Damage and the Mitochondrial Apoptotic Pathway
复制标题
谷胱甘肽通过预防氧化损伤和线粒体凋亡途径减少 HEK293 细胞中棒曲霉素引起的细胞毒性
DOI：
10.1021/acs.jafc.8b01212
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发表时间：
2018-07-25
影响因子：
6.1
通讯作者：
Peng, Xiaoli
中科院分区：
文献类型：
--
作者：
Wang, Xiaorui;Jin, Chengni;Peng, Xiaoli
棒曲霉素（PAT）是一种经常在发霉的水果和水果制品中检测到的真菌毒素。本研究探讨了谷胱甘肽（GSH），一种抗氧化剂，对PAT诱导的细胞毒性的保护作用及其在HEK293细胞中的潜在机制。结果表明，GSH的加入显著提高了细胞活力，降低了PAT诱导的细胞凋亡。此外，GSH还能降低细胞内ROS和线粒体ROS的过量产生，抑制线粒体膜电位的下降，维持细胞内ATP含量。GSH可防止线粒体氧化磷酸化系统的损伤，尤其是提高电子传递链复合物III（UQCRC 2）和复合物V（ATP 5、ATP 6和ATP 8）的mRNA和蛋白水平。此外，GSH增加内源GSH含量，提高SOD，CAT，GR和GPx的抗氧化酶活性，并调制氧化损伤。这些结果表明，GSH通过抑制HEK293细胞中的氧化损伤和线粒体凋亡途径来降低PAT诱导的细胞毒性。
Patulin (PAT) is a mycotoxin frequently detected in moldy fruits and fruit products. This study investigated the protective role of glutathione (GSH), an antioxidant agent, against PAT-induced cytotoxicity and its potential mechanisms in HEK293 cells. The obtained results showed that the addition of GSH significantly increased cell viability and decreased apoptosis induced by PAT. Additionally, GSH decreased intracellular ROS and mitochondrial ROS overproduction, suppressed the decline of the mitochondrial membrane potential, and maintained cellular ATP contents. GSH prevented the impairment of mitochondrial oxidative-phosphorylation system and, especially, enhanced the mRNA and protein levels of electron-transportchain complex III (UQCRC2) and complex V (ATP5, ATP6 and ATP8). Furthermore, GSH increased endogenous GSH contents; enhanced the antioxidant-enzyme activities of SOD, CAT, GR, and GPx; and modulated oxidative damage. These results suggest that GSH reduces PAT-induced cytotoxicity via inhibition of oxidative damage and the mitochondrial apoptotic pathway in HEK293 cells.