Identification of eight homeobox-containing transcripts expressed during larval development and at metamorphosis in the gastropod mollusc Haliotis rufescens.
Identification of eight homeobox-containing transcripts expressed during larval development and at metamorphosis in the gastropod mollusc Haliotis rufescens.
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发表时间：
1993
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通讯作者：
B. Degnan;D. Morse
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作者：
B. Degnan;D. Morse
红鲍鱼（Haliotis rufescens）是一种特别容易处理的实验模型，用于研究发生在变态过程中的分子和细胞变化，因为浮游幼虫的同步沉降和变态可以在实验室中诱导。利用退化引物逆转录聚合酶链反应，以果蝇触角类同源结构域一致序列为基础，鉴定了8个不同转录的同源结构域。这是从软体动物门分离出来的第一批同源箱形动物。提出的部分同源盒序列中有5个似乎代表了假定的haaliotis homc /Hox复合体的触角样基因；其余的显示出与不同类别的同源盒的相似性。对早期发育、幼虫和变态阶段的RNA分析表明，触角样同源盒转录物存在于trochophore和veliger幼虫以及处于变态阶段的定居个体中，但不存在于卵或早期胚胎中。
The red abalone, Haliotis rufescens, is an especially tractable experimental model for studying molecular and cellular changes that occur at metamorphosis, because synchronous settlement and metamorphosis of the planktonic larvae can be induced in the laboratory. Using reverse transcription-polymerase chain reaction with degenerate primers based on the Drosophila Antennapedia-class homeodomain consensus sequence, eight differentially transcribed homeoboxes were identified from Haliotis larvae prior to and during metamorphosis. These are the first homeoboxes isolated from the phylum Molusca. Five of the partial homeobox sequences presented appear to represent Antennapedia-like genes of the putative Haliotis HOM-C/Hox complex; the remainder show similarity to homeoboxes of divergent classes. Analysis of RNA from a range of early developmental, larval, and metamorphic stages revealed that Antennapedia-like homeobox transcripts are present in trochophore and veliger larvae and in settled individuals undergoing metamorphosis, but not in the egg or early embryo.