Biogeography and environmental genomics of the Roseobacter-affiliated pelagic CHAB-I-5 lineage
Biogeography and environmental genomics of the Roseobacter-affiliated pelagic CHAB-I-5 lineage
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DOI：
10.1038/nmicrobiol.2016.63
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发表时间：
2016-07-01
影响因子：
28.3
通讯作者：
Simon, Meinhard
中科院分区：
文献类型：
--
作者：
Billerbeck, Sara;Wemheuer, Bernd;Simon, Meinhard
微生物群落的鉴定和功能表征仍然是微生物海洋学的一个热门话题，因为与环境相关的浮游原核生物的信息仍然有限。Roseflora组是海洋α变形菌门的一个丰富谱系，可以构成浮游细菌的大部分。玫瑰杆菌也与真核生物体相关，并且具有从2.2到>5 Mpb的流线型以及更大的基因组。在这里，我们表明，这一组，CHAB-I-5，发生在全球范围内从热带到极地地区，占到22%的活跃的北海浮游细菌在夏季。CHAB-I-5生物体的第一个测序基因组包含3.6 Mbp，并表现出贫营养生活方式的特征。在北海表面沃茨的元转录组中，存在98%的编码基因，并且编码各种ABC转运蛋白、谷氨酸合酶和CO氧化的基因特别上调。41个基因组的Roseberry组的系统发育基因内容分析揭示了一个独特的集群的远洋生物有别于其他血统的这一组，突出了生活在营养枯竭的环境中的适应。
The identification and functional characterization of microbial communities remains a prevailing topic in microbial oceanography as information on environmentally relevant pelagic prokaryotes is still limited. The Roseobacter group, an abundant lineage of marine Alphaproteobacteria, can constitute large proportions of the bacterioplankton. Roseobacters also occur associated with eukaryotic organisms and possess streamlined as well as larger genomes from 2.2 to >5 Mpb. Here, we show that one pelagic cluster of this group, CHAB-I-5, occurs globally from tropical to polar regions and accounts for up to 22% of the active North Sea bacterioplankton in the summer. The first sequenced genome of a CHAB-I-5 organism comprises 3.6 Mbp and exhibits features of an oligotrophic lifestyle. In a metatranscriptome of North Sea surface waters, 98% of the encoded genes were present, and genes encoding various ABC transporters, glutamate synthase and CO oxidation were particularly upregulated. Phylogenetic gene content analyses of 41 genomes of the Roseobacter group revealed a unique cluster of pelagic organisms distinct from other lineages of this group, highlighting the adaptation to life in nutrient-depleted environments.