Proteomic identification of carbonylated proteins and their oxidation sites.
Proteomic identification of carbonylated proteins and their oxidation sites.
复制标题
DOI：
10.1021/pr1002609
复制
发表时间：
2010-08-06
影响因子：
4.4
通讯作者：
Regnier, Fred E.
中科院分区：
文献类型：
--
作者：
Madian, Ashraf G.;Regnier, Fred E.
关键词：
Excessive oxidative stress leaves a protein carbonylation fingerprint in biological systems. Carbonylation is an irreversible post translational modification (PTM) that often leads to the loss of protein function and can be a component of multiple diseases. Protein carbonyl groups can be generated directly (by amino acids oxidation and the a-amidation pathway) or indirectly by forming adducts with lipid peroxidation products or glycation and advanced glycation end-products. Studies of oxidative stress are complicated by the low concentration of oxidation products and wide array of routes by which proteins are carbonylated. The development of new selection and enrichment techniques coupled with advances in mass spectrometry are allowing identification of hundreds of new carbonylated protein products from a broad range of proteins located at many sites in biological systems. The focus of this review is on the use of proteomics tools and methods to identify oxidized proteins along with specific sites of oxidative damage and the consequences of protein oxidation.
登录
查看更多内容
影响因子：
4.4
作者：
Brock, JWC;Hinton, DJS;Ames, JM
通讯作者：
Ames, JM
影响因子：
2.3
作者：
Aldini, Giancarlo;Gamberoni, Luca;Carini, Marina
通讯作者：
Carini, Marina
影响因子：
4.1
作者：
BRUENNER, BA;JONES, AD;GERMAN, JB
通讯作者：
GERMAN, JB
DOI：
10.1124/jpet.105.088088
发表时间：
2005-10-01
影响因子：
3.5
作者：
Carbone, DL;Doorn, JA;Petersen, DR
通讯作者：
Petersen, DR
影响因子：
2.9
作者：
Cheal, Sarah M.;Ng, Mindy;Meares, Claude F.
通讯作者：
Meares, Claude F.