Malondialdehyde precursors in gamma-irradiated DNA, deoxynucleotides and deoxynucleosides.
Malondialdehyde precursors in gamma-irradiated DNA, deoxynucleotides and deoxynucleosides.
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伽马射线照射的 DNA、脱氧核苷酸和脱氧核苷中的丙二醛前体。
DOI：
10.1093/nar/13.24.9011
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发表时间：
1985
影响因子：
14.9
通讯作者：
Henner,WD
中科院分区：
文献类型：
--
作者：
Janicek,MF;Haseltine,WA;Henner,WD
DNA、脱氧核苷或脱氧核苷酸的伽马辐照产生与硫代巴比妥酸反应的物质，形成在532 nm处具有最大吸光度的发色团。该物质不是丙二醛。我们鉴定了一种新的辐射产物（thyain-1 ′-y1）-丙烯醛作为胸腺嘧啶核苷γ辐射的TBA反应产物。胸腺嘧啶-丙烯醛已被其他学者描述为博来霉素处理DNA的产物。胸苷核苷酸的辐照产生磷酸化的胸腺嘧啶-丙醛前体。对TBA反应性形成条件的研究表明，TBA反应性的形成机制涉及自由基与脱氧核糖核酸分子和分子氧的反应，在此基础上，提出了γ射线照射产生DNA中TBA反应性物质的反应顺序与博莱霉素催化DNA中碱基丙烯醛形成的反应顺序相同。博来霉素和γ-照射的不同之处在于序列进行到完成的程度与游离碱-丙烯醛的释放。
Gamma-irradiation of DNA, deoxynucleosides, or deoxynucleotides produces material that reacts with thiobarbituric acid to form a chromophore with maximum absorbance at 532 nm. This material is not malondialdehyde. We have identified a new radiation product (thyain-1′-y1)-propenal as the TBA-reactive product of gamma-irradiation of thymidine. Thymine-propenal has been described by other inveitigators as a product of bleomycin-treatment of DNA. Irradiation of thymidine nuclaotides produces phosphorylated precursors to thymine-propanal. Studies of the requirements for formation of TBA-reactivity indicate a mechanism involving reaction of a free radical with the deoxyriboae moiaty and molecular oxygen.On the basia of these reaults it is proposed that gamma-irradiation produces TBA-reactive matarial in DNA by the same reaction sequence in which bleomycin catalyzes the formation of base-propenals in DNA. Blaomycin and gamaa-irradiation differ in the extent to which the sequence proceeds to completion with release of free base-propenals.