TREATMENT OF ALVEOLAR MACROPHAGES WITH CYTOCHALASIN-D INHIBITS UPTAKE AND SUBSEQUENT GROWTH OF LEGIONELLA-PNEUMOPHILA
TREATMENT OF ALVEOLAR MACROPHAGES WITH CYTOCHALASIN-D INHIBITS UPTAKE AND SUBSEQUENT GROWTH OF LEGIONELLA-PNEUMOPHILA
复制标题
DOI：
10.1128/iai.51.1.31-36.1986
复制
发表时间：
1986-01-01
影响因子：
3.1
通讯作者：
WINN, WC
中科院分区：
文献类型：
--
作者：
ELLIOTT, JA;WINN, WC
嗜肺军团菌在豚鼠和大鼠肺泡巨噬细胞中迅速繁殖，但当用0.5或1.0 μ g/ml细胞松弛素D预处理抑制吞噬活性时，不能生长。细胞松弛素D不抑制粘附。L.嗜肺菌非常接近有活力的功能性巨噬细胞，或者甚至当细菌附着在巨噬细胞的质膜上时。非调理L.嗜肺细胞被肺泡巨噬细胞贪婪地吞噬。当细菌离心到细胞团块上时，超过85%的吞噬细胞在15分钟内含有一种或多种细菌。相反，在相同条件下，只有大约15%的巨噬细胞含有nonopsonized大肠杆菌或金黄色葡萄球菌。L.的吞噬作用未处理的豚鼠巨噬细胞的嗜肺性通过以经典方式在细菌周围延伸伪足而发生。细菌不能主动穿透吞噬细胞表明，它们的细胞内存活不一定依赖于避免吞噬体，而是依赖于对巨噬细胞随后的杀微生物功能的抑制或抵抗。
Legionella pneumophila multiplied rapidly in guinea pig and rat alveolar macrophages but failed to grow when phagocytic activity was inhibited by pretreatment with 0.5 or 1.0 .mu.g of cytochalasin D per ml. Attachment was not inhibited by cytochalasin D. No extracellular multiplication occurred when L. pneumophila were in close proximity to viable functional macrophages or even when the bacteria were attached to plasma membranes of the macrophges. Nonopsonized L. pneumophila were avidly phagocytized by alveolar macrophages. When bacteria were centrifugated onto a cell pellet, more than 85% of the phagocytes contained one or more bacteria within 15 min. In contrast, under the same conditions only approximately 15% of the macrophages contained nonopsonized Escherichia coli or Staphylococcus aureus. Phagocytosis of L. pneumophila by untreated guinea pig macrophages occurred by extension of pseudopodia around the bacteria in a classical manner. The failure of the bacteria to actively penetrate the phagocyte suggests that their intracellular suvival must not depend on avoidance of a phagosome but rather on an inhibition of or resistance to subsequent microbicidal functions of the macrophage.