SITE-DIRECTED MUTAGENESIS OF THE GAG-MYC GENE OF AVIAN MYELOCYTOMATOSIS VIRUS-29 - BIOLOGICAL-ACTIVITY AND INTRACELLULAR-LOCALIZATION OF STRUCTURALLY ALTERED PROTEINS
SITE-DIRECTED MUTAGENESIS OF THE GAG-MYC GENE OF AVIAN MYELOCYTOMATOSIS VIRUS-29 - BIOLOGICAL-ACTIVITY AND INTRACELLULAR-LOCALIZATION OF STRUCTURALLY ALTERED PROTEINS
复制标题
DOI：
10.1128/jvi.60.1.167-176.1986
复制
发表时间：
1986-10-01
影响因子：
5.4
通讯作者：
PARSONS, JT
中科院分区：
文献类型：
--
作者：
HEANEY, ML;PIERCE, J;PARSONS, JT
将鸡肉胚细胞用PMC29，一种质粒载体转染，其中包含急性转化病毒MC29序列的质粒载体，以及克隆的转化转化缺陷辅助辅助病毒，P.Delta.mst，导致形态学转化，P110GAG-MYC的合成（产品） Gag-myc癌基因）和传染病的产生。 MC29突变体在插孔特异性序列内或MYC序列的中部内有效地导向缺失，从而有效诱导了培养物中鸡胚细胞的转化。但是，在MYC基因的氨基末端半末端或羧基末端部分中包含序列缺失的变体对于转化有缺陷。这些变体有效地定位于细胞核编码的GAG-MYC蛋白。分离过过早的终止突变体，该突变体编码了缺乏羧基末端185残基的GAG-MYC蛋白；这些截断的蛋白质局部局部局部和细胞质。在MYC特异性序列中间（残基ILE-239至GLU-249）中，删除少于11个残基可显着降低鸡造血细胞转化的效率。
Transfection of chicken embryo cells with pMC29, a plasmid vector containing the sequences for the acute transforming virus MC29, and a cloned transformation-defective helper virus, p.DELTA.Mst, resulted in morphological transformation, the synthesis of P110gag-myc (the product of the gag-myc oncogene), and the production of infectious virus. MC29 mutants bearing site-directed deletions within the gag-specific sequences or within the middle portion of the myc sequences efficiently induced transformation of chicken embryo cells in culture. However, variants containing deletions of sequences in the amino-terminal half or carboxy-terminal portion of the myc gene were defective for transformation. The gag-myc proteins encoded by these variants efficiently localized to the cell nucleus. Premature termination mutants were isolated which encoded gag-myc proteins lacking the carboxy-terminal 185 residues; these truncated proteins localized to both the nucleus and the cytoplasm. Deletion of as few as 11 residues within the middle of the myc-specific sequences (residues Ile-239 to Glu-249) significantly reduced the efficiency of chicken hematopoietic cell transformation.