Formation and analysis of topographical domains between lipid membranes tethered by DNA hybrids of different lengths
Formation and analysis of topographical domains between lipid membranes tethered by DNA hybrids of different lengths
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DOI：
10.1039/c2fd20108a
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发表时间：
2013-01-01
影响因子：
3.4
通讯作者：
Boxer, Steven G.
中科院分区：
文献类型：
--
作者：
Chung, Minsub;Koo, Bon Jun;Boxer, Steven G.
我们最近描述了一种制备DNA系留的脂质膜的策略，以将DNA固定在表面上或将DNA展示在支持的双层上[Boxer等人，J. Struct. Biol.，2009，168，190; Boxer等人，Langmuir，2011，27，5492]。对于后一种系统，DNA杂交体是横向移动的;当使用不同长度的正交正义-反义对时，DNA杂交体通过高度分离，并且系留膜变形以适应高度差。这种结构对于模拟由分子识别介导的膜之间的相互作用特别有用，并且类似于细胞与细胞的连接。膜之间的DNA杂交体的长度、亲和力和数量是完全可控的。有趣的模式高度偏析观察荧光干涉显微镜。在偏析和斑图形成过程中观察到不同的行为，并讨论了可能的机制。这个模型系统捕获了突触形成的一些基本物理过程，是理解细胞间连接中脂质膜行为的一个步骤。
We recently described a strategy to prepare DNA-tethered lipid membranes either to fixed DNA on a surface or to DNA displayed on a supported bilayer [Boxer et al., J. Struct. Biol., 2009, 168, 190; Boxer et al., Langmuir, 2011, 27, 5492]. With the latter system, the DNA hybrids are laterally mobile; when orthogonal sense-antisense pairs of different lengths are used, the DNA hybrids segregate by height and the tethered membrane deforms to accommodate the height difference. This architecture is particularly useful for modelling interactions between membranes mediated by molecular recognition and resembles cell-to-cell junctions. The length, affinity and population of the DNA hybrids between the membranes are completely controllable. Interesting patterns of height segregation are observed by fluorescence interference contrast microscopy. Diverse behavior is observed in the segregation and pattern forming process and possible mechanisms are discussed. This model system captures some of the essential physics of synapse formation and is a step towards understanding lipid membrane behaviour in cell-to-cell junctions.