HIGH-RESOLUTION ANALYSIS OF MODIFIED QUARTER-STAGGER MODEL OF COLLAGEN FIBRIL
HIGH-RESOLUTION ANALYSIS OF MODIFIED QUARTER-STAGGER MODEL OF COLLAGEN FIBRIL
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DOI：
10.1002/bip.1974.360130509
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发表时间：
1974-01-01
期刊：
影响因子：
2.9
通讯作者：
GROSS, J
中科院分区：
文献类型：
--
作者：
BRUNS, R;GROSS, J
在阳性染色胶原原纤维的主要重复周期内的横向带的位置是通过光学平均程序从电子显微照片确定的。根据这些数据和SLS晶体中已发表的条带位置，我们准备了一个二维表示，精确到约25 Å，胶原纤维中分子的修饰四分之一交错排列。有了这些信息，就有可能证明纤维内单个胶原分子上的基因座之间的关系。例如，胶原蛋白分子被蝌蚪胶原酶切割的位点、双糖单元与α1 - CB5肽共价结合的位点、羧基末端分子间交联的位点都发生在“空穴区”氨基末端边缘附近的纤维中；氨基末端交联的位点发生在“空穴区”的羧基末端边缘附近。
The location of transverse bands within the major repeating period of positively stained collagen fibrils was determined from electron micrographs by an optical averaging procedure. From these data and the published location of bands in SLS crystallites, we have prepared a two‐dimensional representation, accurate to about 25 Å, of the modified quarter‐stagger arrangement of molecules in the collagen fibril. With this information it is possible to demonstrate the relationship of loci on individual collagen molecules within the fibril. For example, the site where the collagen molecule is cleaved by tadpole collagenase, the site where a disaccharide unit is covalently bound to the α1‐CB5 peptide, and the site of carboxyl‐terminal intermolecular cross‐linking all occur in the fibril near the amino‐terminal edge of the “hole zone;” and the site of amino‐terminal cross‐linking occurs near the carboxyl‐terminal edge of the “hole zone.”