Transcriptional response of human umbilical vein endothelial cell to H9N2 influenza virus infection
Transcriptional response of human umbilical vein endothelial cell to H9N2 influenza virus infection
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人脐静脉内皮细胞对H9N2流感病毒感染的转录反应
DOI：
10.1016/j.virol.2015.03.037
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Qiao Jian
中科院分区：
文献类型：
--
作者：
Wang Wei;Mu Xiang;Zhao Lihong;Wang Jianfang;Chu Yaocheng;Feng Xuejian;Feng Bo;Wang Xiaohong;Zhang Jianjun;Qiao Jian
内皮细胞被认为在应答病毒感染中起重要作用。在这里，我们使用微阵列技术来研究基因表达谱在人脐静脉内皮细胞在感染后24小时与H9N2病毒或灭活的H9N2病毒颗粒。结果表明，H9N2病毒感染诱导了大量的差异表达基因，表现出病毒感染的转录特征。治疗后检测到高水平的趋化因子基因表达。令人惊讶的是，最显著上调的基因主要是干扰素刺激基因（ISG），尽管干扰素基因表达和干扰素蛋白水平没有变化。我们还发现病毒颗粒在诱导ISGs表达方面比病毒更有效。这些结果表明，诱导ISGs的表达主要依赖于病毒颗粒和内皮细胞之间的相互作用。我们的数据为内皮细胞和H9N2流感病毒之间的相互作用提供了进一步的见解。
Endothelial cells are believed to play an important role in response to virus infection. Here, we used a microarray technology to study the gene expression profile in human umbilical vein endothelial cells at 24 h postinfection with H9N2 viruses or inactivated H9N2 viral particles. The results showed that H9N2 virus infection induced an abundance of differential expressed genes, exhibiting a transcriptional signature of viral infection. High levels of chemokine gene expressions were detected following treatment. Surprisingly, the most significantly up-regulated genes were mainly interferon-stimulated genes (ISGs), although there was no change in interferon gene expression and interferon protein level. We also found that viral particles were more potent than viruses in inducing ISGs expression. These results suggest that induction of expression of ISGs is mainly dependent on the interaction between viral particles and endothelial cells. Our data offer further insight into the interaction between endothelial cells and H9N2 influenza viruses.