The Effects of Low-Dose-Rate γ-irradiation on Forced Swim Test-Induced Immobility and Oxidative Stress in Mice
The Effects of Low-Dose-Rate γ-irradiation on Forced Swim Test-Induced Immobility and Oxidative Stress in Mice
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DOI：
10.18926/amo/61896
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发表时间：
2021-04-01
影响因子：
0.5
通讯作者：
Yamaoka, Kiyonori
中科院分区：
文献类型：
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作者：
Nakada, Tetsuya;Kataoka, Takahiro;Yamaoka, Kiyonori
强迫游泳测试（FST）诱导小鼠不动。低剂量（高剂量率）X 射线辐射因其抗氧化功能而抑制 FST 诱导的小鼠不动。我们评估了低剂量率的低剂量伽马射线照射对 FST 诱导的小鼠器官抗氧化剂消耗的影响。小鼠接受全身低剂量率（0.6 或 3.0 mGy/h）低剂量伽马射线照射 1 周，然后每日进行 FST（5 天）。与第一天相比，3.0 mGy/h 照射小鼠的第 2 天不动率显着低于假照射小鼠。 FST 分别显着降低了大脑和肾脏中的过氧化氢酶（CAT）活性和总谷胱甘肽（t-GSH）含量。 3.0 mGy/h照射小鼠肝脏超氧化物歧化酶(SOD)活性和t-GSH含量显着低于未接受FST处理的小鼠。暴露于 3.0 mGy/h 伽玛射线照射的小鼠肺部的 CAT 活性高于未接受 FST 治疗的小鼠和接受 FST 治疗的小鼠。然而，除了肺部的 CAT 活性外，假手术组和辐射组之间这些抗氧化标志物的水平没有观察到显着差异。这些发现表明低剂量率和低剂量伽马射线照射对 FST 的影响高度依赖于器官。
The forced swim test (FST) induces immobility in mice. Low-dose (high-dose-rate) X-irradiation inhibits FST-induced immobility in mice due to its antioxidative function. We evaluated the effects of low-dose gamma-irradiation at a low-dose-rate on the FST-induced depletion of antioxidants in mouse organs. Mice received whole-body low-dose-rate (0.6 or 3.0 mGy/h) of low-dose gamma-irradiation for 1 week, followed by daily FSTs (5 days). The immobility rate on day 2 compared to day 1 was significantly lower in the 3.0 mGy/h irradiated mice than in sham irradiated mice. The FST significantly decreased the catalase (CAT) activity and total glutathione (t-GSH) content in the brain and kidney, respectively. The superoxide dismutase (SOD) activity and t-GSH content in the liver of the 3.0 mGy/h irradiated mice were significantly lower than those of the non-FST-treated mice. The CAT activity in the lungs of mice exposed to 3.0 mGy/h gamma-irradiation was higher than that of non-FST treated mice and mice treated with FST. However, no significant differences were observed in the levels of these antioxidant markers between the sham and irradiated groups except for the CAT activity in lungs. These findings suggest that the effects of low-dose-rate and low-dose gamma-irradiation on FST are highly organ-dependent.