Total plasma homocysteine correlates in women with gestational diabetes
Total plasma homocysteine correlates in women with gestational diabetes
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DOI：
10.1007/s00404-008-0571-1
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发表时间：
2008-10-01
影响因子：
2.6
通讯作者：
Sieradzki, J.
中科院分区：
文献类型：
--
作者：
Idzior-Walus, B.;Cyganek, K.;Sieradzki, J.
目的探讨妊娠期糖尿病（GDM）孕妇血清总同型半胱氨酸（tHcy）水平与代谢综合征（MS）和B族维生素的关系。使用Bio-Rad试剂通过ELISA分析血清同型半胱氨酸水平。血清叶酸和维生素B-12浓度测定荧光免疫法，游离脂肪酸（FFA）和lipidemase.Results血清同型半胱氨酸水平在GDM和CG组相似（8 +/- 2.0与7.4 +/- 1.1 μ mol/L，分别）。与对照组相比，GDM妇女的胰岛素抵抗稳态模型（HOMA-IR）值较高。（2.8 ± 1.7 vs 1.6 ± 0.9，P < 0.01），血清甘油三酯（2.7 +/- 0.9 vs 1.9 +/- 0.5 mmol/l，P < 0.01）和FFA（0.6 +/- 0.2 vs 0.46 +/-0.2 mmol/l，P < 0.05）。GDM组血清tHcy水平与维生素B 12（r =-0.47，P < 0.01）、叶酸（r =-0.51，P < 0.001）、胰岛素抵抗指标HOMA-IR（r =-0.49，P < 0.05）相关。在以血清tHcy为因变量的多元回归分析中，叶酸和维生素B-12进入GDM妇女的分析（β分别为-0.42和-0.34，P < 0.05），而在CG中，胱抑素C和HOMA-IR进入分析结论GDM患者血清同型半胱氨酸水平与维生素B-12和叶酸水平显著相关，而健康孕妇血清同型半胱氨酸水平与HOMA-IR和肾功能显著相关。结果表明，B族维生素在调节胰岛素抵抗/妊娠期糖尿病妇女血清tHcy水平中的重要性，这可能与保护GDM妇女免受与tHcy升高相关的妊娠并发症有关。
Aim We aim to assess serum total homocysteine (tHcy) associations with metabolic syndrome components and B-vitamins in women with gestational diabetes mellitus (GDM).Methods We studied 61 consecutive pregnant women, 44 with GDM and 17 with normal glucose tolerance (CG). Serum homocysteine levels were analyzed by ELISA, using Bio-Rad reagents. Serum folates and vitamin B-12 concentrations were determined by chemiluminescent immunoassay, free fatty acids (FFA) and lipids enzymatically.Results Serum homocysteine levels were similar in both the GDM and the CG groups (8 +/- 2.0 vs 7.4 +/- 1.1 mu mol/l, respectively). Women with GDM in comparison to CG women were characterized by higher values of homeostasis model of insulin resistance (HOMA-IR) (2.8 +/- 1.7 vs 1.6 +/- 0.9, P < 0.01), serum triglycerides (2.7 +/- 0.9 vs 1.9 +/- 0.5 mmol/l, P < 0.01) and FFA (0.6 +/- 0.2 vs 0.46 +/- 0.2 mmol/l, P < 0.05). In GDM women serum tHcy correlated with vitamin B-12 (r = -0.47, P < 0.01) and folates (r = -0.51, P < 0.001); in CG women with HOMA-IR, a marker of insulin resistance (r = -0.49, P < 0.05). In multiple regression analysis with serum tHcy as a dependent variable, folate and vitamin B-12 entered the analysis in GDM women (beta = -0.42 and -0.34, respectively, P < 0.05), whereas in CG cystatin C and HOMA-IR entered the analysis (P < 0.05).Conclusions In women with GDM, serum homocysteine is significantly associated with vitamin B-12 and folate levels, while in healthy pregnant women with HOMA-IR and with kidney function. The results suggest the importance of the B-group vitamins in regulation of serum tHcy levels in women with insulin resistance/gestational diabetes, what might be relevant in protection against pregnancy complications associated with elevated tHcy in GDM women.