Viable circulating metastatic cells produced in orthotopic but not ectopic prostate cancer models.
Viable circulating metastatic cells produced in orthotopic but not ectopic prostate cancer models.
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DOI：
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发表时间：
2003-07
期刊：
影响因子：
11.2
通讯作者：
A. Glinskii;Brian A. Smith;P. Jiang;Xiaoming Li;Meng Yang;R. Hoffman;G. Glinsky
中科院分区：
文献类型：
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作者：
A. Glinskii;Brian A. Smith;P. Jiang;Xiaoming Li;Meng Yang;R. Hoffman;G. Glinsky
阐明非依赖性转移性前列腺癌的机制仍然是一个重要的和高度相关的挑战。我们在这里报告，原位生长的难治性人前列腺癌有效地提供了可行的转移细胞在宿主循环。这与相同谱系的异位肿瘤相反，异位肿瘤不将活细胞递送到循环中。为了研究存活的循环癌细胞的恶性潜能，我们开发了一种新的人前列腺癌转移的裸鼠双色原位共植入模型。该模型由共注射分离和培养的循环绿色荧光蛋白表达克隆和亲本红色荧光蛋白表达人前列腺癌细胞的等效混合物组成。在双色模型中，选择的绿色荧光蛋白标记的活循环细胞相对于红色荧光蛋白标记的亲本细胞具有增加的转移倾向。从原位而非异位模型中鉴定和分离高度恶性的活循环人前列腺癌细胞将使我们对转移过程（包括肿瘤微环境的作用）有重要的新认识。
Elucidation of the mechanisms of hormone-independent metastatic prostate cancer remains a significant and highly relevant challenge. We report here that hormone-refractory human prostate carcinoma growing orthotopically efficiently deliver viable metastatic cells in the host circulation. This is in contrast to the ectopic tumors of the same lineage, which do not deliver live cells into the circulation. To investigate the malignant potential of viable circulating carcinoma cells, we developed a novel dual-color orthotopic coimplantation model of human prostate cancer metastasis in nude mice. This model is comprised of coinjection of an equivalent mixture of isolated and cultured circulating green fluorescent protein-expressing clones and parental red fluorescent protein-expressing human prostate carcinoma cells. In the dual-color model, the selected green fluorescent protein-labeled viable circulating cells have an increased metastatic propensity relative to the red fluorescent protein-labeled parental cells. The identification and isolation of highly malignant viable circulating human prostate carcinoma cells from orthotopic but not ectopic models will enable important new insights into the metastatic process including the role of the tumor microenvironment.