Hormonal stimulation of erythropoietin production and erythropoiesis in anephric sheep fetuses.
Hormonal stimulation of erythropoietin production and erythropoiesis in anephric sheep fetuses.
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无肾羊胎儿促红细胞生成素产生和红细胞生成的激素刺激。
DOI：
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发表时间：
1979
影响因子：
15.9
通讯作者：
M. Banisadre
中科院分区：
文献类型：
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作者：
E. Zanjani;M. Banisadre
本实验研究了睾酮（DT）和甲状腺素（L-T_4）对正常和肾切除绵羊胎仔红细胞生成和促红细胞生成素（Ep）的影响。对胎仔注射1.2 mg/d x 13 L-T4、12 mg DT（每5 d一次x 3）或单独溶媒。在给药开始前和给药开始后间隔时间采集用于Ep测定的胎仔血浆样本。使用网织红细胞和红细胞压积水平以及红细胞-59 Fe摄取百分比值评估每只胎仔的红细胞生成。对照胎仔血浆中未检测到Ep，而DT和L-T4处理的完整胎仔血浆中存在大量Ep。双侧肾切除术并没有减少对DT和L-T4的Ep反应。在完整和肾切除胎儿，DT治疗导致生产显着更大量的Ep比L-T4。所有组的Ep升高均伴随网织红细胞（2.2 +/- 0.2% vs L-T4：8.1 +/- 0.4%和DT：7.6 +/- 0.7%）、红细胞-59 Fe摄取百分比（20.5 +/- 2.9% vs L-T4：36.7 +/- 3.8%和DT：39.1 +/- 4.0%）和红细胞压积（31.2 +/- 2% vs L-T4：41.8 +/- 3%和DT：48.6 +/- 4.2%）增加。增强红细胞生成在所有组的肾切除胎儿是依赖于Ep的存在，因为抗Ep的管理，这些胎儿导致在所有三个组的红细胞生成的抑制。这些数据表明，（a）DT和L-T4是肾外Ep产生的有效促进剂，从而增强完整和肾切除胎儿的红细胞生成;（B）DT是比L-T4更强的肾外Ep形成刺激物;和（c）Ep是L-T4和DT的红细胞生成作用表达所必需的。
The effect of testosterone (DT) and thyroxin (L-T4) on erythropoiesis and erythropoietin (Ep) production was studied in control and nephrectomized sheep fetuses beginning at about 100 d of gestation. Fetuses were given injections of either 1.2 mg/d x 13 of L-T4, 12 mg, once every 5 d x 3 of DT or the vehicle alone. Fetal plasma samples for Ep determinations were obtained before and at intervals after the start of the treatment. Reticulocyte and hematocrit levels, and the percent erythrocyte-59Fe uptake values were used to assess erythropoiesis in each fetus. No Ep was detected in plasmas of control fetuses, while significant amounts of Ep were present in plasma obtained from DT- and L-T4-treated intact fetuses. Bilateral nephrectomy did not diminish the Ep response to DT and L-T4. In both intact and nephrectomized fetuses, treatment with DT resulted in the production of significantly greater amounts of Ep than L-T4. The rise in Ep in all groups was accompanied by increases in reticulocytes (2.2 +/- 0.2% vs L-T4:8.1 +/- 0.4% and DT:7.6 +/- 0.7%), percent erythrocyte-59Fe uptake (20.5 +/- 2.9% vs. L-T4:36.7 +/- 3.8% and DT:39.1 +/- 4.0%) and hematocrit (31.2 +/- 2% vs. L-T4:41.8 +/- 3% and DT:48.6 +/- 4.2%). The enhanced erythropoiesis in all groups of nephrectomized fetuses was dependent upon the presence of Ep, because the administration of anti-Ep to these fetuses resulted in the suppression of erythropoiesis in all three groups. These data demonstrate that (a) DT and L-T4 are effective promoters of extrarenal Ep production, thereby enhancing erythropoiesis in intact and nephrectomized fetuses; (b) DT is a stronger stimulus of extrarenal Ep formation than L-T4; and (c) Ep is required for the expression of the erythropoietic effects of L-T4 and DT.