Reactivation at low ATP distinguishes among classes of paralyzed flagella mutants.
Reactivation at low ATP distinguishes among classes of paralyzed flagella mutants.
复制标题
低 ATP 条件下的重新激活可区分不同类别的麻痹鞭毛突变体。
DOI：
10.1002/(sici)1097-0169(1997)38:1
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发表时间：
1997
影响因子：
--
通讯作者：
Omoto,CK
中科院分区：
文献类型：
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作者：
Frey,E;Brokaw,CJ;Omoto,CK
衣原体鞭毛麻痹突变体的特征是完整的细胞不能运动。这些突变体的脱膜鞭毛在标准条件下用毫摩尔ATP浓度重新激活时也是不动的。先前发现这些突变体中的三个在3种替代的再活化条件下再活化时是能动的：低ATP浓度（> 50 μM）; 0.1 mM ATP与>0.5 mM ADP组合;或0.1 mM ATP与非再活化ATP类似物邻氨基苯甲酰ATP或甲基邻氨基苯甲酰ATP组合。我们现在已经调查了衣原体培养物保藏中心的所有pf突变体，发现这些突变体中的绝大多数可以在这些交替的核苷酸条件下移动。只有pf 22和pf 23，动力蛋白臂缺失多个亚群的突变体，在这些条件下没有重新激活。这表明在大多数pf突变体中观察到的麻痹是生理ATP抑制的结果。除了pf 12具有异常对称的弯曲模式外，所有其他pf突变体都表现出与野生型相似的不对称弯曲模式。以前，发现通过抑制突变的存在而恢复的运动性缺乏野生型鞭毛或抑制子本身的正常不对称性。pf突变体在不存在抑制子的交替再激活条件下的波形显示，具有径向辐条或中心对缺陷的pf突变体能够与野生型类似地不对称弯曲。一个完整的径向辐条/中心对复合体对于非对称弯曲模式的产生并不重要。此外，这表明以前在抑制型pf突变体中观察到的对称波形是由于pf和抑制突变之间的相互作用。细胞动力。Cytoskeleton 38：91-99，1997.© 1997 Wiley利斯公司
Paralyzed flagella(pf) mutants ofChlamydomonashave been distinguished by the inability of the intact cells to move. Demembranated flagella from these mutants are also immotile when reactivated under standard conditions, with millimolar ATP concentrations. Three of thesepfmutants were previously found to be motile when reactivated under 3 alternate reactivation conditions: low ATP concentration (≦50 μM); 0.1 mM ATP combined with >0.5 mM ADP; or 0.1 mM ATP combined with non‐reactivating ATP analogs anthraniloyl ATP or methylanthraniloyl ATP. We have now surveyed allpfmutants in theChlamydomonasCulture Collection and discovered that a great majority of these mutants can move under these alternate nucleotide conditions. Onlypf22andpf23,mutants missing multiple subsets of dynein arms, did not reactivate under those conditions. This suggests that the paralysis observed in mostpfmutants is the result of inhibition by physiological ATP. Except forpf12,which has an abnormally symmetric bending pattern, all otherpfmutants exhibit asymmetric bending patterns similar to wild‐type. Previously, motility that was restored by the presence of suppressor mutations was found to lack the normal asymmetry of wild‐type flagella or the suppressor by itself. The waveform ofpfmutants at alternate reactivation conditions in the absence of suppressor shows thatpfmutants with radial‐spoke or central‐pair defects are capable of asymmetric bending similar to wild‐type. A complete radial‐spoke/central‐pair complex is not essential for the production of asymmetric bending patterns. Furthermore, this suggests that the symmetric waveform observed previously in suppressedpfmutants is due to the interaction between thepfand suppressor mutations. Cell Motil. Cytoskeleton 38:91–99, 1997. © 1997 Wiley‐Liss, Inc.