Arabidopsis NIP3;1 Plays an Important Role in Arsenic Uptake and Root-to-Shoot Translocation under Arsenite Stress Conditions.
Arabidopsis NIP3;1 Plays an Important Role in Arsenic Uptake and Root-to-Shoot Translocation under Arsenite Stress Conditions.
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DOI：
10.1016/j.molp.2015.01.005
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发表时间：
2015-05
期刊：
影响因子：
27.5
通讯作者：
Wenzhong Xu;Wentao Dai;Huili Yan;Sheng Li;Hongling Shen;Yanshan Chen;Hua Xu;Yangyang Sun
中科院分区：
文献类型：
--
作者：
Wenzhong Xu;Wentao Dai;Huili Yan;Sheng Li;Hongling Shen;Yanshan Chen;Hua Xu;Yangyang Sun
在拟南芥中，水通道蛋白的结节蛋白26样内在蛋白（NIP）亚家族由9个成员组成，其中5个（NIP1;1、NIP1;2、NIP5;1、NIP6；1和NIP7；1）先前已被鉴定为可渗透亚砷酸盐。然而，NIPs在植物中亚砷酸盐从根到茎的转运中的作用仍然知之甚少。在本研究中，利用反向遗传策略，拟南芥NIP3；1在亚砷胁迫条件下对砷的吸收和根到梢的分布都起重要作用。Thenip3;功能缺失突变体对地上生长的亚砷酸盐耐受性有明显提高，枝条中砷的积累量低于野生型，而功能缺失突变体对地上生长的亚砷酸盐耐受性明显高于野生型；1 nip1;双突变体表现出较强的亚砷酸盐耐受性，在亚砷酸盐胁迫条件下，根和芽的生长均有所改善。启动子-β-葡萄糖醛酸酶分析显示nip3；1几乎完全在根中表达（根尖除外），而在酵母（酿酒酵母）中的异源表达表明，NIP3；1能够介导亚砷酸盐的运输。综上所述，我们的结果表明NIP3；1参与拟南芥对亚砷酸盐的吸收和根到茎的转运，可能是一个被动的双向亚砷酸盐转运体。
InArabidopsis, the nodulin 26-like intrinsic protein (NIP) subfamily of aquaporin proteins consists of nine members, five of which (NIP1;1, NIP1;2, NIP5;1, NIP6;1, and NIP7;1) were previously identified to be permeable to arsenite. However, the roles of NIPs in the root-to-shoot translocation of arsenite in plants remain poorly understood. In this study, using reverse genetic strategies,Arabidopsis NIP3;1was identified to play an important role in both the arsenic uptake and root-to-shoot distribution under arsenite stress conditions. Thenip3;1loss-of-function mutants displayed obvious improvements in arsenite tolerance for aboveground growth and accumulated less arsenic in shoots than those of the wild-type plants, whereas thenip3;1 nip1;1double mutant showed strong arsenite tolerance and improved growth of both roots and shoots under arsenite stress conditions. A promoter-β-glucuronidase analysis revealed thatNIP3;1was expressed almost exclusively in roots (with the exception of the root tips), and heterologous expression in the yeastSaccharomyces cerevisiaedemonstrated that NIP3;1 was able to mediate arsenite transport. Taken together, our results suggest that NIP3;1 is involved in arsenite uptake and root-to-shoot translocation inArabidopsis, probably as a passive and bidirectional arsenite transporter.