STUDIES ON HUMAN PORIN .13. THE TYPE-1 VDAC PORIN 31HL BIOTINYLATED AT THE PLASMALEMMA OF TRYPAN BLUE EXCLUDING HUMAN B-LYMPHOCYTES
STUDIES ON HUMAN PORIN .13. THE TYPE-1 VDAC PORIN 31HL BIOTINYLATED AT THE PLASMALEMMA OF TRYPAN BLUE EXCLUDING HUMAN B-LYMPHOCYTES
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DOI：
10.1016/0014-5793(95)00465-l
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发表时间：
1995-07-10
期刊：
影响因子：
3.5
通讯作者：
HILSCHMANN, N
中科院分区：
文献类型：
--
作者：
JAKOB, C;GOTZ, H;HILSCHMANN, N
1989年，我们首次证明了VDAC 'Porin 31 HL'在人B淋巴细胞质膜中的表达，然后首次证明了VDAC的多拓扑表达，同时，来自本实验室和其他实验室的数据支持我们关于哺乳动物组织中通道的多室分布的建议。随后通过二维电泳、免疫和链霉亲和素亲和印迹，我们表明部分通道分子可以在细胞外膜上被标记。因此，通过相关的方法，我们的结果推翻了关于抗人1型孔蛋白抗体与非VDAC蛋白在细胞外膜上的假定交叉反应性的异议。
In 1989, we demonstrated for the first time the expression of the VDAC 'Porin 31HL' in the plasmalemma of human B lymphocytes, then giving first evidence of a multi-topological expression of VDAC, Meanwhile, data from this and other laboratories support our proposal of a multi-compartment distribution of the channel in mammalian tissues, Here, by biotinylation of plasmalemma-integrated proteins of proven living B lymphocytes, followed by two-dimensional electrophoresis, immuno- and streptavidin affinity blotting, we show that part of the channel molecules can be labelled at the outer membrane of the cells, Thus, by a relevant approach our results invalidate objections concerning putative cross-reactivity of anti-human Type-1 porin antibodies with non-VDAC proteins at the outer cell membrane.