Cell wall hydroxycinnamates in wild rice (Zizania aquatica L.) insoluble dietary fibre
Cell wall hydroxycinnamates in wild rice (Zizania aquatica L.) insoluble dietary fibre
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DOI：
10.1007/s00217-002-0512-3
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发表时间：
2002-06-01
影响因子：
3.3
通讯作者：
Steinhart, H
中科院分区：
文献类型：
--
作者：
Bunzel, M;Allerdings, E;Steinhart, H
研究了野生稻(Zizania Aquatica L.)中酯键酚酸的含量。通过高效液相分析定量膳食纤维，并分离和鉴定低聚糖羟基肉桂酸酯，以研究羟基肉桂酸与细胞壁聚合物的联系。野生稻不溶性膳食纤维(WRIDF)中，阿魏酸是含量最高的酚酸(3942µg(-1))，还含有大量的芥子酸(518µg(-1))和对香豆酸(142µg(-1))。用碳水化合物水解酶或三氟乙酸处理WRIDF会释放出几种低聚糖羟基肉桂酸酯。经Amberlite XAD-2柱层析分离得到5个阿魏酸化低聚糖，经凝胶层析和半制备高效液相色谱分离鉴定为阿拉伯木聚糖阿魏酸酯片段。阿魏酸化四糖{[5-O-(trans-feruloyl)][O-beta-D-Xylp-(1-->；2)]-O-alpha-L-Araf-(1-->；3)}-O-beta-D-Xylp-(1-->；4)-D-Xylp为首次从食用植物原料中分离得到。虽然一些结果表明芥子酸也(至少部分地)通过酯键与多糖相连，但没有实现明确的低聚糖芥子酸酯的分离。
The contents of ester-linked phenolic acids in wild rice (Zizania aquatica L.) dietary fibre were quantified by HPLC analysis, and oligosaccharide hydroxycinnamates were isolated and identified to investigate the linkages of hydroxycinnamic acids to cell wall polymers. In wild rice insoluble dietary fibre (WRIDF), ferulic acid was the most abundant phenolic acid (3942 mug g(-1)), but significant amounts of sinapic acid (518 mug g(-1)) and p-coumaric acid (142 mug g(-1)) were also detected. Treatment of WRIDF with carbohydrate hydrolases or trifluoroacetic acid released several oligosaccharide hydroxycinnamates. After fractionation with Amberlite XAD-2, five feruloylated oligosaccharides were isolated by gel chromatography and semi-preparative HPLC and identified as arabinoxylan ferulate fragments. The feruloylated tetrasaccharide {[5-O-(trans-feruloyl)][O-beta-D-Xylp-(1-->2)]-O-alpha-L-Araf-(1-->3)}-O-beta-D-Xylp-(1-->4)-D-Xylp was isolated for the first time from edible plant material. Although some results indicated that sinapic acid is also (at least partially) linked through an ester bond to polysaccharides, isolation of defined oligosaccharide sinapates was not achieved.