Identification and fine mapping of alien fragments associated with enhanced grain weight from Agropyron cristatum chromosome 7P in common wheat backgrounds
Identification and fine mapping of alien fragments associated with enhanced grain weight from Agropyron cristatum chromosome 7P in common wheat backgrounds
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DOI：
10.1007/s00122-021-03927-7
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发表时间：
2021-08
影响因子：
5.4
通讯作者：
Yangyang Sun;Mingjie Lyu;Hai-ming Han;Shenghui Zhou;Yuqing Lu;Weihua Liu;Xin-ming Yang;Xiuquan Li-Xiu
中科院分区：
文献类型：
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作者：
Yangyang Sun;Mingjie Lyu;Hai-ming Han;Shenghui Zhou;Yuqing Lu;Weihua Liu;Xin-ming Yang;Xiuquan Li-Xiu
关键信息定位于7PS1-2区域的冰草7p染色体上存在一个粒重增强的基因座，位于两个小麦背景中。利用该基因座对新的易位系进行了鉴定。摘要冰草是小麦的野生近缘种，具有优良的小麦改良基因。小麦-A。Cristatum7P二体附加系II-5-1具有较高的粒重。在这里，我们来剖析Ya对粒重贡献的遗传基础。在小麦背景中，开发了附加系的四个分离群体，并在两个小麦背景下对其进行了评估。结果表明，这是一种有效的方法。在小麦不同粒重水平下，7p染色体能稳定显著增加粒重约2g，主要是通过增加粒长实现的。7P染色体上的粒重增加基因座表现出与小麦背景无关的显性遗传。用细胞学方法和分子标记鉴定了2个缺失系和23个易位系，构建了由158个STS标记和40个碱基间隔组成的7P染色体扩增图。利用这些缺失和易位系的遗传群体，粒重增加的遗传座位被缩小到bin 7PS1-2。7p染色体片段较小的两个易位系(7pt-A18和7pt-B4)的粒重、粒长和粒宽同时增加。这些携带7PS1-2染色体片段的易位系将成为小麦粒重改良的宝贵遗传资源。总体而言，本研究发现了7P染色体上的粒重基因，并提供了新的粒重增强的预育系。
Key messageAn enhanced grain weight locus fromAgropyron cristatumchromosome 7P was verified in two wheat backgrounds, localized to the 7PS1-2 region. Novel translocation lines with this locus were evaluated.AbstractAgropyron cristatumis a wild relative of wheat that harbours elite genes for wheat improvement. The wheat-A. cristatum7P disomic addition line II-5-1 exhibits high grain weight. Here, to dissect the genetic basis of grain weight contributed byA. cristatumchromosome 7P in wheat backgrounds, four segregated populations of the addition line were developed and evaluated in two wheat backgrounds. The results showed thatA. cristatumchromosome 7P can stably and significantly increase the grain weight by approximately 2 g, mainly by increasing grain length at different grain weight levels of the wheat background. The locus for increased grain weight from chromosome 7P shows dominant inheritance independent of the wheat background. Moreover, two deletion lines and 23 translocation lines were identified by cytological methods and molecular markers, and an enlarged chromosome 7P bin map was constructed with 158 STS markers and 40 bin intervals. With the genetic populations of these deletion and translocation lines, the genetic locus of increased grain weight was narrowed down to bin 7PS1-2. Two translocation lines (7PT-A18 and 7PT-B4) with smaller 7P chromosomal segments exhibited an increase in grain weight, grain length and grain width simultaneously. These translocation lines carrying the 7PS1-2 chromosomal fragment will be valuable genetic resources for wheat grain weight improvement. Collectively, this study uncovers the grain weight locus from chromosome 7P and provides novel pre-breeding lines with enhanced grain weight.