The mouse epididymal transcriptome: Transcriptional profiling of segmental gene expression in the epididymis
The mouse epididymal transcriptome: Transcriptional profiling of segmental gene expression in the epididymis
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DOI：
10.1095/biolreprod.105.039719
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发表时间：
2005-09-01
影响因子：
3.6
通讯作者：
Turner, TT
中科院分区：
文献类型：
--
作者：
Johnston, DS;Jelinsky, SA;Turner, TT
精子的成熟，包括获得运动能力和经历获能的能力，发生在通过附睾的动态环境的运输过程中。沿着附睾小管沿着长度形成的微环境对于精子的分子修饰是必不可少的，这些分子修饰导致可育配子。这些微环境是由排列在小管上的上皮细胞的分泌和吸收过程产生的。在本研究中，10个形态学上不同的部分小鼠附睾进行了鉴定显微解剖。我们假设附睾腔环境的变化是由这些片段之间的差异基因表达建立的。通过微阵列分析来分析从10个片段中的每一个分离的RNA。在小鼠附睾中表达的基因超过17000个，而从整个附睾样品中鉴定的基因约为12000个。筛选一组正常小鼠组织，鉴定了附睾选择性和附睾特异性转录物。此外，该研究还鉴定了2168个基因，这些基因在至少两个不同片段之间上调或下调超过4倍。这些基因的表达模式识别不同的节段调控模式。利用主成分分析，我们确定这10个片段形成6个不同的转录单位。这些分析阐明了基因表达的变化沿着长度的附睾为17000表达的成绩单，并提供了一个强大的资源，为研究界在未来的研究，介导附睾精子成熟的生物因素。
Maturation of spermatozoa, including the acquisition of motility and the ability to undergo capacitation, occurs during transit through the dynamic environment of the epididymis. The microenvironments created along the length of the epididymal tubule are essential to the molecular modifications of spermatozoa that result in fertile gametes. The secretory and resorptive processes of the epithelial cells that line this tubule generate these microenvironments. In the current study, 10 morphologically distinct segments of the mouse epididymis were identified by microdissection. We hypothesized that the changing environments of the epididymal lumen are established by differential gene expression among these segments. RNA isolated from each of the 10 segments was analyzed by microarray analysis. More than 17 000 genes are expressed in the mouse epididymis, compared with about 12 000 genes identified from whole epididymal samples. Screening a panel of normal mouse tissues identified both epididymal-selective and epididymal-specific transcripts. In addition, this study identified 2168 genes that are up-regulated or down-regulated by greater than 4-fold between at least two different segments. The expression patterns of these genes identify distinct patterns of segmental regulation. Using principal component analysis, we determined that the 10 segments form 6 different transcriptional units. These analyses elucidate the changes in gene expression along the length of the epididymis for 17000 expressed transcripts and provide a powerful resource for the research community in future studies of the biological factors that mediate epididymal sperm maturation.