FIBER AND SYNAPTIC CONNECTIONS BETWEEN EMBRYONIC RETINAL TRANSPLANTS AND HOST RETINA
FIBER AND SYNAPTIC CONNECTIONS BETWEEN EMBRYONIC RETINAL TRANSPLANTS AND HOST RETINA
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DOI：
10.1006/exnr.1995.1027
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发表时间：
1995-06-01
影响因子：
5.3
通讯作者：
SEILER, MJ
中科院分区：
文献类型：
--
作者：
ARAMANT, RB;SEILER, MJ
本研究的目的是调查（a）胚胎视网膜移植是否可以发芽纤维到受损的成人宿主视网膜和（B），如果这些纤维建立与主机的突触连接。将胚胎大鼠（E16-22）或人（9-13周）视网膜细胞移植到成年大鼠中。正常的Long-Evans大鼠接受了大鼠移植。人体移植的宿主是无胸腺裸鼠。在不同的存活时间（3至11个月）后，用4%多聚甲醛（有时添加0.1%戊二醛）灌注动物。将涂有DiI（一种碳菁染料）的玻璃微针放入移植物中，然后将其在室温下在2%多聚甲醛中储存3-15个月。这充满了在针被放置的区域中具有突起的细胞。在振动切片机上切割明胶包埋的眼杯。DiI标记的移植细胞表现出纤维长入宿主视网膜。在将染料光转化为电子致密沉淀物后，这些神经过程可以比荧光更好的分辨率进行跟踪。偶尔，宿主细胞也可以被DiI标记到移植物中，表明宿主纤维向内生长到移植物中。将选择的光转化切片包埋用于电子显微镜检查。突触可以发现沿着移植过程已经生长到宿主的内丛状层。这些结果表明，源自胚胎视网膜移植物的神经元纤维在宿主视网膜中形成突触。(C)出版社：Academic Press
The aim of this study was to investigate (a) whether embryonic retinal transplants can sprout fibers into a lesioned adult host retina and (b) if these fibers established synaptic connections with the host. Embryonic rat (E16-22) or human (9-13 weeks) retinal cells were transplanted to adult rats. Normal Long-Evans rats received rat transplants. The hosts for human transplants were athymic nude rats. After varying survival times (3 to 11 months), animals were perfused with 4% paraformaldehyde (sometimes with added 0.1% glutaraldehyde). Glass microneedles, coated with DiI (a carbocyanine dye) were placed into the transplants which were then stored at room temperature in 2% paraformaldehyde for 3-15 months. This filled the cells that had processes in the area where the needle had been placed. Gelatin-embedded eyecups were cut on a vibratome. DiI-labeled transplant cells exhibited fiber outgrowth into the host retina. After photoconversion of the dye to an electron-dense precipitate, these neuronal processes could be followed with better resolution than with fluorescence. Occasionally, host cells could also be labeled by DiI placed into the graft; indicating fiber ingrowth of host fibers into the transplants. Selected photoconverted sections were embedded for electron microscopy. Synapses could be found along transplant processes that had grown into the host inner plexiform layer. These results indicate that neuronal fibers originating from embryonic retinal transplants form synapses in the host retina. (C) 1995 Academic Press, Inc.