Discovery and Validation of Circulating Hsa-miR-210-3p as a Potential Biomarker for Primary Open-Angle Glaucoma
Discovery and Validation of Circulating Hsa-miR-210-3p as a Potential Biomarker for Primary Open-Angle Glaucoma
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循环 Hsa-miR-210-3p 作为原发性开角型青光眼潜在生物标志物的发现和验证
DOI：
10.1167/iovs.19-26663
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发表时间：
2019-07-01
影响因子：
4.4
通讯作者：
Zhang, Xiulan
中科院分区：
文献类型：
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作者：
Liu, Yaoming;Wang, Yayi;Zhang, Xiulan
目的。目前，在临床实践中尚无基于血液的青光眼诊断工具，可用于在无法利用传统眼科检查时筛查患者。本研究旨在鉴定与原发性开角型青光眼 (POAG) 相关的循环 microRNA (miRNA)，并探索它们作为诊断标记物的实用性。共有 136 名 POAG 患者和对照组入组。使用新一代 RNA 测序来探索测序组中循环 miRNA 的表达谱，并通过定量实时聚合酶链式反应 (qRT-PCR) 验证来自筛选组和验证组中独立样本的潜在 miRNA。接受者操作特征 (ROC) 分析用于评估某些 miRNA 区分 POAG 患者和对照受试者的能力。 结果。使用测序和 qRT-PCR，发现所有 POAG 患者中 hsa-miR-210-3p 均升高。筛选和验证集的 ROC 分析显示，hsa-miR210-3p 在 POAG 患者和匹配对照之间进行区分，曲线下面积 (AUC) 分别为 0.846（敏感性：84.6%；特异性：80.8%）和 0.813（敏感性：84.8%；特异性：69.7%）。对于所有非测序参与者，分析显示 hsa-miR-210-3p 区分严重 POAG 患者和对照组的 AUC 为 0.880（敏感性：85.4%；特异性：85.7%）。此外，hsa-miR-210-3p的表达与j平均偏差j（β = 0.237；P = 0.022）和平均视网膜神经纤维层厚度（β = -5.792；P = 0.014）的视野缺陷相关。结论。循环hsa-miR-210-3p可以作为POAG的潜在诊断标志物（特别是对于严重的POAG患者）。
PURPOSE. Blood-based examination tools for glaucoma diagnosis in clinical practice, which can be useful for screening patients when traditional ophthalmic examinations cannot be utilized, are not available thus far. This study aimed to identify circulating microRNAs (miRNAs) associated with primary open-angle glaucoma (POAG) and explore their utility as diagnostic markers.METHODS. A total of 136 POAG patients and controls were enrolled. Next-generation RNA sequencing was used to explore the expression profile of circulating miRNAs in the sequencing set, and potential miRNAs from independent samples in both the screening and validation sets were validated by quantitative real-time polymerase chain reaction (qRT-PCR). Receiver operating characteristic (ROC) analysis was used to evaluate the ability of certain miRNAs to distinguish POAG patients from control subjects.RESULTS. Using sequencing and qRT-PCR, hsa-miR-210-3p was found to be elevated in POAG patients in all sets. ROC analysis of the screening and validation sets revealed that hsa-miR210-3p differentiated between POAG patients and matched controls with an area under the curve (AUC) of 0.846 (sensitivity: 84.6%; specificity: 80.8%) and 0.813 (sensitivity: 84.8%; specificity: 69.7%), respectively. In case of all nonsequencing participants, analysis revealed that hsa-miR-210-3p differentiated between severe POAG patients and controls with an AUC of 0.880 (sensitivity: 85.4%; specificity: 85.7%). In addition, the expression of hsa-miR-210-3p was associated with visual field defects of jmean deviationj (beta = 0.237; P = 0.022) and average retinal nerve fiber layer thickness (beta = -5.792; P = 0.014).CONCLUSIONS. Circulating hsa-miR-210-3p may serve as a potential diagnostic marker for POAG (especially for severe POAG patients).