Microtubule competition and cell growth recenter the nucleus after anaphase in fission yeast.
Microtubule competition and cell growth recenter the nucleus after anaphase in fission yeast.
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DOI：
10.1091/mbc.e23-01-0034
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发表时间：
2023-07-01
影响因子：
3.3
通讯作者：
Laplante, Caroline
中科院分区：
文献类型：
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作者：
Bellingham-Johnstun, Kimberly;Thorn, Annelise;Belmonte, Julio M.;Laplante, Caroline
细胞根据它们的活动主动定位它们的细胞核。在分裂酵母中，依赖于微管的核中心对于细胞的对称分裂是至关重要的。在后期纺锤体解体后，细胞核在∼90分钟内重新中心，大约是细胞周期持续时间的一半。活细胞和模拟实验支持两种不同的微管竞争机制在细胞核缓慢重新定位过程中的协同作用。首先，推-推机制从纺锤体拆解到分裂，涉及有丝分裂的纺锤体极体微管的相反动作，将细胞核推离细胞末端，而后期的微管阵列将细胞核装入篮子，限制其向分裂平面的迁移。其次，一种缓慢生长的机制通过微管竞争和不对称细胞生长的组合慢慢地将细胞核集中在新生细胞中。我们的工作强调了微管的固有属性如何根据微管网络组织和细胞大小而不同地影响核定位。
Cells actively position their nuclei based on their activity. In fission yeast, microtubule-dependent nuclear centering is critical for symmetrical cell division. After spindle disassembly at the end of anaphase, the nucleus recenters over an ∼90-min period, approximately half of the duration of the cell cycle. Live-cell and simulation experiments support the cooperation of two distinct microtubule competition mechanisms in the slow recentering of the nucleus. First, a push–push mechanism acts from spindle disassembly to septation and involves the opposing actions of the mitotic spindle pole body microtubules that push the nucleus away from the ends of the cell, while a postanaphase array of microtubules baskets the nucleus and limits its migration toward the division plane. Second, a slow-and-grow mechanism slowly centers the nucleus in the newborn cell by a combination of microtubule competition and asymmetric cell growth. Our work underlines how intrinsic properties of microtubules differently impact nuclear positioning according to microtubule network organization and cell size.
DOI：
10.1083/jcb.200403090
发表时间：
2004-06-07
期刊：
The Journal of cell biology
影响因子：
--
作者：
Feierbach B;Verde F;Chang F
通讯作者：
Chang F