Primer RNA for DNA synthesis on single-stranded DNA template in a cell free system from Drosophila melanogaster embryos.
Primer RNA for DNA synthesis on single-stranded DNA template in a cell free system from Drosophila melanogaster embryos.
复制标题
用于在来自果蝇胚胎的无细胞系统中的单链 DNA 模板上合成 DNA 的引物 RNA。
DOI：
10.1093/nar/11.11.3433
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发表时间：
1983
影响因子：
14.9
通讯作者：
T. Okazaki
中科院分区：
文献类型：
--
作者：
K. Yoda;T. Okazaki
A cytoplasmic extract of Drosophila melanogaster early embryos supported DNA synthesis which was dependent on an added single stranded DNA template, phi X174 viral DNA. The product DNA made during early reaction was about 100 to 600 nucleotides in length and complementary to the added template. After alkali treatment, 70 to 80 per cent of the product DNA chains exposed 5'-hydroxyl ends, suggesting covalent linkage of primer RNA at their 5'-ends. Post-labeling of 5'-ends of the product DNA with polynucleotide kinase and [gamma-32P]ATP revealed that oligoribonucleotides, mainly hexa- and heptanucleotides, were covalently linked to the 5'-ends of the majority of the DNA chains. The nucleotide sequence of the linked RNA was mainly 5'(p)ppApA(prN)4-5, where tri- (or di-) phosphate terminus was detected by the acceptor activity for the cap structure with guanylyltransferase and [alpha-32P]GTP. The structure of this primer RNA was comparable to that of the octaribonucleotide primer isolated from the nuclei of Drosophila early embryos.
