The rtPA increases MMP-9 activity in serum during ischaemic stroke
The rtPA increases MMP-9 activity in serum during ischaemic stroke
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DOI：
10.1016/j.pjnns.2014.07.012
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发表时间：
2014-01-01
影响因子：
2.9
通讯作者：
Kurzepa, Jacek
中科院分区：
文献类型：
--
作者：
Golab, Piotr;Boguszewska-Czubara, Anna;Kurzepa, Jacek
背景和目的：目的：探讨急性缺血性脑卒中（ischaemic stroke，IS）患者血清中MMP-9、MMP-3和TIMP-1水平与rtPA治疗的关系。rtPA组14例，给予Actylise溶栓治疗。rtPA组在3个时间点采集血清样本（时间点0：卒中后第1 - 4小时;时间点1 -rtPA给药后即刻;时间点2 -卒中发作后第5-7天）。其余患者在两个时间点进行静脉采血：卒中第1 - 5 - 10小时时间点和卒中第5-7天时间点2。明胶酶谱法检测MMP-9，ELISA法检测血清MMP-3和TIMP-1水平。NIHSS改善率（IR）计算为入院和出院时NIHSS评分之间的差异。结果：在所有分析的样本中均未观察到MMP-9（86 kDa）的活性形式。与非rtPA组相比，rtPA组中总MMP-9活性在时间点1显著升高。与非rtPA组相比，rtPA给药期间MMP-3血清水平显著降低，并在时间点2恢复。结论：溶栓治疗IS可提高血清MMP-9活性，而rtPA不能促进MMP-9前体向活性形式的转化。我们的研究结果还表明，MMP-3参与的生化过程中发生的急性期IS。(C)2014年波兰神经学会。出版社：Elsevier Urban & Partner Sp. z o.o. All rights reserved.
Background and purpose: To find the relationship between rtPA treatment vs. MMP-9 activity, MMP-3, and TIMP-1 serum levels related to patients neurological status during acute ischaemic stroke (IS).Material and methods: 35 IS patients were enrolled. 14 of them underwent thrombolytic therapy with Actylise (rtPA group). The serum samples were obtained at 3 time-points for rtPA group (time-point 0: 1st-4th hour of stroke; time-point 1 - immediately after rtPA administration; time-point 2 - on day 5-7 from stroke onset). Remaining patients had venous blood collection at two time-points: time-point 1 - 5th-10th hour of stroke and time-point 2 on day 5-7 of stroke. MMP-9 was analyzed with gelatin zymography, MMP-3 and TIMP-1 serum levels were analyzed with ELISA method. NIHSS improvement ratio (IR) was calculated as a difference between NIHSS score at the admission and discharge of patient.Results: The active form of MMP-9 (86 kDa) was not observed in any analyzed samples. Total MMP-9 activity was significantly elevated at time-point 1 in rtPA group in comparison with non-rtPA group. MMP-3 serum level significantly decreased during rtPA administration in comparison with non-rtPA group and it was restored at time-point 2. MMP-3 negatively correlated with IR values (p = 0.06).Conclusions: Thrombolysis applied for IS treatment increases MMP-9 activity in serum, however, rtPA does not facilitate the conversion of pro-MMP-9 into the active form. Our results also suggest the involvement of MMP-3 to the biochemical processes occurring during acute phase of IS. (C) 2014 Polish Neurological Society. Published by Elsevier Urban & Partner Sp. z o.o. All rights reserved.