Identification and mapping of resistance to stem rust in the European winter wheat cultivars Spark and Rialto
Identification and mapping of resistance to stem rust in the European winter wheat cultivars Spark and Rialto
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DOI：
10.1007/s11032-016-0537-0
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发表时间：
2016-08-01
影响因子：
3.1
通讯作者：
Park, Robert F.
中科院分区：
文献类型：
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作者：
Getie, Banchgize;Singh, Davinder;Park, Robert F.
以英国冬小麦品种Spark和Rialto杂交获得的126个双单倍体（DH）系为作图群体，研究了它们对小麦条锈菌（Puccinia graminis f.）在温室和人工接种的田间小区，在两个地点超过3年（2011年，2012年和2013年）。遗传分析表明，参与两个幼苗基因（Sr 5和Sr 31，由Rialto贡献）和三个成年植物抗性基因。对田间试验数据的QTL分析表明，染色体臂1BS（Rialto贡献）、3BS和5A（Spark贡献）上的3个效应QTL参与了对秆锈病的抗性。这些QTL分别解释了78.5%、9.0%和5.9%的表型变异。在田间条件下，Sr 5的毒力存在，Sr 31的毒力缺失，在1BS上检测到的QTL（QSr.sun-1BS）归属于主效苗期抗病基因Sr 31。位于染色体臂3BS上的QTL（QSr.sun-3BS）与SSR标记gwm 1034紧密连锁，位于染色体臂5A上的QTL（QSr.sun-5A）与SSR标记gwm 443紧密连锁。携带QSr.sun-3BS和QSr. sun-5A表现出较低的茎锈病反应，表明两个APR基因在降低疾病严重性方面的加性效应。本研究所获得的标记可用于小麦抗秆锈QTL与其它主效或微效基因的连锁分析和标记辅助选择。
A mapping population of 126 doubled haploid (DH) lines derived from a cross between the English winter wheat cultivars Spark and Rialto was evaluated for response to Puccinia graminis f. sp. tritici in the greenhouse and in artificially inoculated field plots at two locations over 3 years (2011, 2012 and 2013). Genetic analysis indicated the involvement of two seedling genes (Sr5 and Sr31, contributed by Rialto) and three adult plant resistance genes. QTL analyses of field data showed the involvement of three consistent effects QTL on chromosome arms 1BS (contributed by Rialto), and 3BS and chromosome 5A (contributed by Spark) in the observed resistance to stem rust. These QTLs explained average phenotypic variation of 78.5, 9.0 and 5.9 %, respectively. With the presence of virulence for Sr5 and absence of Sr31 virulence in the field, the QTL detected on 1BS (QSr.sun-1BS) was attributed to the major seedling resistance gene Sr31. The QTL located on chromosome arm 3BS (QSr.sun-3BS) was closely associated with SSR marker gwm1034, and the QTL detected on 5A (QSr.sun-5A) was closely linked with SSR marker gwm443. DH lines carrying the combination of QSr.sun-3BS and QSr. sun-5A exhibited lower stem rust responses indicating the additive effects of the two APR genes in reducing disease severity. The markers identified in this study can be useful in pyramiding these QTLs with other major or minor genes and marker assisted selection for stem rust resistance in wheat.