Plasma Xanthine Oxidoreductase Activity as a Novel Biomarker of Metabolic Disorders in a General Population
Plasma Xanthine Oxidoreductase Activity as a Novel Biomarker of Metabolic Disorders in a General Population
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DOI：
10.1253/circj.cj-18-0082
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发表时间：
2018-07-01
影响因子：
3.3
通讯作者：
Miura, Tetsuji
中科院分区：
文献类型：
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作者：
Furuhashi, Masato;Matsumoto, Megumi;Miura, Tetsuji
背景：黄嘌呤氧化还原酶（XOR）是一种催化次黄嘌呤和黄嘌呤形成尿酸的酶，导致超氧化物和活性氧的增加。激活XOR促进氧化应激相关的组织损伤。我们研究了代谢参数与血浆XOR活性之间的关系，采用液相色谱和三重四极杆质谱相结合的方法，以[C-13(2),N-15(2)]-黄嘌呤为底物检测[C-13(2),N-15(2)]-尿酸。方法和结果：从Tanno-Sobetsu研究中招募了627名日本受试者（M/F, 292/335），这是一项基于人群的队列研究。血浆XOR活性男性明显高于女性，而习惯性吸烟与活性升高有关。血浆XOR活性与体重指数（BMI）呈正相关；r= 0.323， P
Background: Xanthine oxidoreductase (XOR) is an enzyme that catalyzes the formation of uric acid from hypoxanthine and xanthine, leading to an increase in superoxide and reactive oxygen species. Activation of XOR promotes oxidative stress-related tissue injury. We investigated the associations between metabolic parameters and plasma XOR activity measured by a sensitive and accurate assay using a combination of liquid chromatography and triple quadrupole mass spectrometry to detect [C-13(2),N-15(2)]-uric acid using [C-13(2),N-15(2)]-xanthine as a substrate.Methods and Results: A total of 627 Japanese subjects (M/F, 292/335) from the Tanno-Sobetsu Study, a population-based cohort, were recruited. Plasma XOR activity was significantly higher in males than in females, and habitual smoking was associated with elevation of activity. Plasma XOR activity was positively correlated with body mass index (BMI; r= 0.323, P