Novel function of Wsc proteins as a methanol-sensing machinery in the yeast Pichia pastoris
Novel function of Wsc proteins as a methanol-sensing machinery in the yeast Pichia pastoris
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DOI：
10.1111/mmi.13631
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发表时间：
2017-04-01
影响因子：
3.6
通讯作者：
Sakai, Yasuyoshi
中科院分区：
文献类型：
--
作者：
Ohsawa, Shin;Yurimoto, Hiroya;Sakai, Yasuyoshi
Wsc家族蛋白是从酵母到哺乳动物细胞都保守存在的跨质膜传感蛋白。我们研究了Wsc家族蛋白在甲醇营养型酵母Pichia pastoris中的功能作用，发现PpWsc 1和PpWsc 3在甲醇生长过程中起甲醇传感器的作用。PpWsc 1对甲醇浓度的响应范围比PpWsc 3低。PpWsc1，而不是PpWsc3，也在高温胁迫下发挥作用，但PpWsc1的传感甲醇作为一个信号，是不同于高温胁迫。我们还发现，已知在细胞壁完整性途径中Wsc家族蛋白下游起作用的PpRom2也参与感测甲醇。基于这些结果，这些PpWsc家族蛋白被证明参与感测甲醇并通过PpRom 2将信号经由其胞质尾传递到细胞核，PpRom 2在调节甲醇诱导基因的子集的表达以协调良好平衡的甲醇代谢中起关键作用。
Wsc family proteins are plasma membrane spanning sensor proteins conserved from yeasts to mammalian cells. We studied the functional roles of Wsc family proteins in the methylotrophic yeast Pichia pastoris, and found that PpWsc1 and PpWsc3 function as methanol-sensors during growth on methanol. PpWsc1 responds to a lower range of methanol concentrations than PpWsc3. PpWsc1, but not PpWsc3, also functions during high temperature stress, but PpWsc1 senses methanol as a signal that is distinct from high-temperature stress. We also found that PpRom2, which is known to function downstream of the Wsc family proteins in the cell wall integrity pathway, was also involved in sensing methanol. Based on these results, these PpWsc family proteins were demonstrated to be involved in sensing methanol and transmitting the signal via their cytoplasmic tail to the nucleus via PpRom2, which plays a critical role in regulating expression of a subset of methanol-inducible genes to coordinate well-balanced methanol metabolism.