Neutrophil-anti-microbial interaction in the established infection:: Effect on Staphylococcus aureus
Neutrophil-anti-microbial interaction in the established infection:: Effect on Staphylococcus aureus
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DOI：
10.1016/j.jinf.2005.07.017
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发表时间：
2006-05-01
影响因子：
28.2
通讯作者：
Herndon, Betty L.
中科院分区：
文献类型：
--
作者：
Koomer, Ajoy;Quinn, Tim;Herndon, Betty L.
目的：为了确定是否差异药物相关的金黄色葡萄球菌杀死，在体内与中性粒细胞减少症，是嗜中性粒细胞相关的体外，这种相互作用的机制。金黄色葡萄球菌相互作用，分离细胞壁酶，PBPs，并在与嗜中性粒细胞孵育（或不孵育）后评价它们与五种（β内酰胺和其它）抗生素的结合。S.在无菌过滤的脓肿液的生长培养基中测定试验药物对金黄色葡萄球菌的杀灭作用，所述脓肿液是青霉素感染的或正常感染的。在供试分离株的MBC处，将每种药物或生理盐水对照与S. aureus 106和稀释平板培养。aureus消除了S.金黄色葡萄球菌PBP-2条带在所有蛋白质印迹中，无论用于标记PBP的药物如何。S.生长在脓毒症或正常脓肿液中的金黄色葡萄球菌显示出在脓毒症脓肿液中所有药物的更大杀伤力（p=0.029 6小时孵育）。培养基之间的杀伤差异与药物PBP-2活性相关。结论：药物对S.金黄色葡萄球菌在体外通过中性粒细胞孵育而改变。这是一个由S.金黄色葡萄球菌PBP-2药物结合提示新的宿主细菌。相互作用，可能会影响药物治疗的S。金黄色葡萄球菌感染(c)2005年，英国传染病协会。由爱思唯尔有限公司出版。保留所有权利。
Objectives: To determine if differences in drug-related Staphylococcus aureus killing, associated in vivo with neutropenia, is neutrophil-related in vitro, and the mechanisms of this interaction.Methods: To evaluate the influence of living neutrophils on drug-S. aureus interactions, cell wall enzymes, the PBPs, were isolated and their binding to five (beta lactam and other) antibiotics was evaluated following incubation (or not) with neutrophils. S. aureus killing by the test drugs was assayed in growth media of sterile filtered abscess fluid, either neutropenic infected or normal infected. At MBCs for the test isolate, each drug or saline control was incubated with S. aureus 106 and dilution-plated.Results: Neutrophil incubation with S. aureus eliminated the S. aureus PBP-2 band in all Western blots irrespective of the drug used to tag the PBPs. Time-kill of S. aureus grown in neutropenic or normal abscess fluid showed greater kill by all drugs in neutropenic abscess fluid (p=0.029 6 h incubation). Killing difference between the media correlates with drug PBP-2 activity.Conclusions: Drug activity against S. aureus in vitro is changed by neutrophil incubation. The neutrophil-induced toss of S. aureus PBP-2 drug binding suggests novel host-bacterial. interaction that may impinge on drug treatment of S. aureus infections. (c) 2005 The British Infection Society. Published by Elsevier Ltd. All rights reserved.