Optically controllable molecular logic circuits
Optically controllable molecular logic circuits
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DOI：
10.1063/1.4926361
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发表时间：
2015-07
影响因子：
4
通讯作者：
T. Nishimura;Ryoichi Fujii;Y. Ogura;J. Tanida
中科院分区：
文献类型：
--
作者：
T. Nishimura;Ryoichi Fujii;Y. Ogura;J. Tanida
分子逻辑电路是在分子水平上观察和操纵生物系统的一种很有前途的技术。然而，实现用于时间和可编程操作的分子逻辑电路仍然具有挑战性。在这篇文章中，我们展示了一种使用荧光共振能量转移(FRET)进行信号传递的光学可控逻辑电路。基于FRET的信号传递过程受分子和光输入的调节。基于FRET的距离依赖性，基于其分子输入，在DNA纳米结构上形成了执行分子逻辑运算所需的FRET路径。此外，DNA纳米结构上的FRET通路是光学控制的，使用光开关荧光分子来指示所需操作的执行和相关的计时。因此，可以使用外部光信号来控制电路的行为。作为例子，研究了一个能够执行两种不同逻辑运算的分子逻辑电路。该电路包含功能DNA和DNA支架，用于构建两条FRET路径，用于对分子输入执行输入1和输入2以及输入1和非输入3操作。该电路通过跟随光信号产生具有所有可能的输入组合的正确输出。此外，基于光照射控制操作执行时序，并且电路响应依赖于时间的输入。实验结果表明，该电路随着时间光信号的输入而改变所需操作的输出，分子逻辑电路是在分子水平上观察和操纵生物系统的一种很有前途的技术。然而，实现用于时间和可编程操作的分子逻辑电路仍然具有挑战性。在这篇文章中，我们展示了一种使用荧光共振能量转移(FRET)进行信号传递的光学可控逻辑电路。基于FRET的信号传递过程受分子和光输入的调节。基于FRET的距离依赖性，基于其分子输入，在DNA纳米结构上形成了执行分子逻辑运算所需的FRET路径。此外，DNA纳米结构上的FRET通路是光学控制的，使用光开关荧光分子来指示所需操作的执行和相关的计时。因此，可以使用外部光信号来控制电路的行为。作为一个例子，一个分子逻辑电路能够执行两个不同的.
Molecular logic circuits represent a promising technology for observation and manipulation of biological systems at the molecular level. However, the implementation of molecular logic circuits for temporal and programmable operation remains challenging. In this paper, we demonstrate an optically controllable logic circuit that uses fluorescence resonance energy transfer (FRET) for signaling. The FRET-based signaling process is modulated by both molecular and optical inputs. Based on the distance dependence of FRET, the FRET pathways required to execute molecular logic operations are formed on a DNA nanostructure as a circuit based on its molecular inputs. In addition, the FRET pathways on the DNA nanostructure are controlled optically, using photoswitching fluorescent molecules to instruct the execution of the desired operation and the related timings. The behavior of the circuit can thus be controlled using external optical signals. As an example, a molecular logic circuit capable of executing two different logic operations was studied. The circuit contains functional DNAs and a DNA scaffold to construct two FRET routes for executing Input 1 AND Input 2 and Input 1 AND NOT Input 3 operations on molecular inputs. The circuit produced the correct outputs with all possible combinations of the inputs by following the light signals. Moreover, the operation execution timings were controlled based on light irradiation and the circuit responded to time-dependent inputs. The experimental results demonstrate that the circuit changes the output for the required operations following the input of temporal light signals.Molecular logic circuits represent a promising technology for observation and manipulation of biological systems at the molecular level. However, the implementation of molecular logic circuits for temporal and programmable operation remains challenging. In this paper, we demonstrate an optically controllable logic circuit that uses fluorescence resonance energy transfer (FRET) for signaling. The FRET-based signaling process is modulated by both molecular and optical inputs. Based on the distance dependence of FRET, the FRET pathways required to execute molecular logic operations are formed on a DNA nanostructure as a circuit based on its molecular inputs. In addition, the FRET pathways on the DNA nanostructure are controlled optically, using photoswitching fluorescent molecules to instruct the execution of the desired operation and the related timings. The behavior of the circuit can thus be controlled using external optical signals. As an example, a molecular logic circuit capable of executing two differ...