Surface Protein Imprinted Core-Shell Particles for High Selective Lysozyme Recognition Prepared by Reversible Addition-Fragmentation Chain Transfer Strategy
Surface Protein Imprinted Core-Shell Particles for High Selective Lysozyme Recognition Prepared by Reversible Addition-Fragmentation Chain Transfer Strategy
复制标题
通过可逆加成-断裂链转移策略制备用于高选择性溶菌酶识别的表面蛋白印迹核壳颗粒
DOI：
10.1021/am5072783
复制
发表时间：
2014-12-24
影响因子：
9.5
通讯作者：
Zhang, Yukui
中科院分区：
文献类型：
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作者：
Li, Qinran;Yang, Kaiguang;Zhang, Yukui
采用可逆加成-断裂链转移（RAFT）法合成了一种新型的溶菌酶（Lys）表面印迹核壳粒子。由于聚合物壳层的链长可控，这种粒子对蛋白质的识别选择性明显提高。将RAFT起始剂和模板蛋白吸附在二氧化硅颗粒上后，将以甲基丙烯酸和甲基丙烯酸2-羟乙酯为单体，以N，N ′-亚甲基双（丙烯酰胺）为交联剂的预聚合溶液与二氧化硅颗粒混合，并在40 ℃下在水相中通过氧化还原引发进行聚合。聚合后，通过去除模板蛋白，用己基胺破坏二硫酯基团，得到了聚合物链长可控的表面Lyz印迹粒子。赖氨酸印迹颗粒的结合容量可达5.6 mg protein/g材料，印迹因子（IF）为3.7，而对照材料的IF仅为1.6。在60 min内达到吸附平衡。此外，赖氨酸可以选择性地识别的印迹颗粒从四种蛋白质的混合物和鸡蛋白色样品。这些结果表明，通过RAFT策略制备的纳米颗粒有望实现对蛋白质的高选择性识别。
A novel kind of lysozyme (Lys) surface imprinted core-shell particles was synthesized by reversible addition-fragmentation chain transfer (RAFT) strategy. With controllable polymer shell chain length, such particles showed obviously improved selectivity for protein recognition. After the RAFT initial agent and template protein was absorbed on silica particles, the prepolymerization solution, with methacrylic acid and 2-hydroxyethyl methacrylate as the monomers, and N,N'-methylenebis(acrylamide) as the cross-linker, was mixed with the silica particles, and the polymerization was performed at 40 degrees C in aqueous phase through the oxidation-reduction initiation. Ater polymerization, with the template protein removal and destroying dithioester groups with hexylamine, the surface Lyz imprinted particles were obtained with controllable polymer chain length. The binding capacity of the Lys imprinted particles could reach 5.6 mg protein/g material, with the imprinting factor (IF) as 3.7, whereas the IF of the control material prepared without RAFT strategy was only 1.6. The absorption equilibrium could be achieved within 60 min. Moreover, Lys could be selectively recognized by the imprinted particles from both a four-proteins mixture and egg white sample. All these results demonstrated that these particles prepared by RAFT strategy are promising to achieve the protein recognition with high selectivity.