TEX101 is shed from the surface of sperm located in the caput epididymidis of the mouse
TEX101 is shed from the surface of sperm located in the caput epididymidis of the mouse
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DOI：
10.1017/s0967199405003394
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发表时间：
2005-11-01
期刊：
影响因子：
1.7
通讯作者：
Takizawa, T
中科院分区：
文献类型：
--
作者：
Takayama, T;Mishima, T;Takizawa, T
通常认为，细胞间的信号转导是由细胞表面分子介导的，细胞表面分子在精子发生和受精过程中发挥着多种重要的调节作用。最近，我们在小鼠睾丸生殖细胞上发现了一种新的质膜相关蛋白TES101-反应蛋白(TES101RP，或TEX101)。在这项研究中，我们用原位杂交的方法研究了TEX101在成年小鼠睾丸中的表达，并通过免疫组织化学和Western印迹分析检测了TEX101在精子运输过程中的命运。在精母细胞和1-9级精细胞中有特异表达，但在精原细胞中不表达。虽然TEX101蛋白仍然存在于STEP 10-16精子细胞和睾丸精子的细胞表面，但它是从附睾头的附睾精子中脱落出来的。这项研究的结果为生殖细胞特异性TEX101在精子发生和睾丸后精子成熟过程中的表达提供了额外的信息。
It is generally believed that cell-to-cell cross-talk and signal transduction are mediated by cell surface molecules that play diverse and important regulatory roles in spermatogenesis and fertilization. Recently, we identified a novel plasma membrane-associated protein, TES101-reactive protein (TES101RP, or TEX101), on mouse testicular germ cells. In this study, we investigate Tex101 mRNA expression in the adult mouse testis using hi situ hybridization, and we examine the fate of TEX101 during sperm transport by immunohistochemical and Western blot analyses. Tex101 mRNA was expressed in a stage-specific manner in spermatocytes and in step 1-9 spermatids of the testis, but not in spermatogonia. Although the TEX101 protein remained on the cell surfaces of step 10-16 spermatids and testicular sperm, it was shed from epididymal sperm located in the caput epididymidis. The results of this study provide additional information on germ cell-specific TEX101 expression during spermatogenesis and post-testicular sperm maturation.