Effects of botulinum toxin type A on expression of genes in keloid fibroblasts.
Effects of botulinum toxin type A on expression of genes in keloid fibroblasts.
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DOI：
10.1177/1090820x13482938
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发表时间：
2014
影响因子：
2.9
通讯作者：
W. Xiaoxue;C. Xi;Xiao Zhibo
中科院分区：
文献类型：
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作者：
W. Xiaoxue;C. Xi;Xiao Zhibo
背景成纤维细胞侵袭性生长受多种生物因素调控，是瘢痕疙瘩病理生理过程中的关键事件。最近的研究表明，A型肉毒毒素(BoNT-A)可以抑制瘢痕疙瘩的侵袭性生长。然而，其分子机制尚不清楚。目的探讨BoNT-A对瘢痕疙瘩成纤维细胞侵袭性生长相关基因表达的影响。方法利用基因芯片技术，从112个与侵袭性生长相关的基因中，研究BoNT-A对瘢痕疙瘩成纤维细胞信使RNA表达的影响。采用实时定量聚合酶链式反应(qRT-PCR)对芯片结果进行验证。结果基因芯片和定量逆转录聚合酶链式反应显示，经BoNT-A处理的成纤维细胞S100A4基因表达上调，转化生长因子-β1、血管内皮生长因子、基质金属蛋白酶-1和PDGFA基因表达下调。结论BONT-A改变了瘢痕疙瘩成纤维细胞中S100A4、转化生长因子-β-1、血管内皮生长因子、基质金属蛋白酶-1和PDGFA基因的表达水平，为探讨BONT-A的功能和寻找治疗瘢痕疙瘩的新方法提供了有用的线索。
BACKGROUND Invasive growth of fibroblast cells, which is regulated by multiple biological factors, is the key event in the pathophysiology of keloid scars. Recent studies have suggested that botulinum toxin type A (BoNT-A) could inhibit invasive growth of keloids. However, the molecular mechanisms are unknown. OBJECTIVE The authors explore the effect of BoNT-A on the expression of genes relevant to invasive growth in keloid fibroblasts. METHODS With 112 genes that were relevant to invasive growth, the authors utilized microarray analysis to study messenger RNA expression profiles in keloid fibroblasts treated with BoNT-A. Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to confirm the microarray results. RESULTS Analyses from microarray and qRT-PCR revealed that the S100A4 gene was upregulated and that the TGF-β1, VEGF, MMP-1, and PDGFA genes were downregulated in fibroblasts treated with BoNT-A. CONCLUSIONS The BoNT-A altered expression levels of S100A4, TGF-β1, VEGF, MMP-1, and PDGFA genes in keloid fibroblasts provide a useful clue for exploring the function of BoNT-A and finding a novel treatment for keloid scarring.