DIESEL EXHAUST PARTICLES INDUCE LOCAL IGE PRODUCTION IN-VIVO AND ALTER THE PATTERN OF IGE MESSENGER-RNA ISOFORMS
DIESEL EXHAUST PARTICLES INDUCE LOCAL IGE PRODUCTION IN-VIVO AND ALTER THE PATTERN OF IGE MESSENGER-RNA ISOFORMS
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DOI：
10.1172/jci117478
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发表时间：
1994-10-01
影响因子：
15.9
通讯作者：
SAXON, A
中科院分区：
文献类型：
--
作者：
DIAZSANCHEZ, D;DOTSON, AR;SAXON, A
柴油废气颗粒（DEP）已牵连到过敏性气道疾病的发病率增加。我们研究了DEP对局部免疫球蛋白产生的影响，通过使用不同剂量的DEP进行鼻腔刺激，并分析了前后鼻腔灌洗液的局部免疫反应。在使用0.30 mg DEP（相当于洛杉矶平均一天的暴露量）4天后，观察到受试者鼻腔IgE显著升高，但IgG、IgA、IgM或白蛋白没有显著升高。depp增强局部产生IgE的直接证据是，攻毒使灌洗液中分泌IgE的细胞数量从200万分之一增加到10万分之一，但没有改变分泌iga的细胞数量。灌洗细胞中epsilon mRNA的产生也随之增加。此外，DEP改变了由选择性剪接产生的5种不同的epsilon mrna的相对数量，这些mrna编码不同的IgE蛋白。这些结果表明，DEP暴露在体内会引起局部IgE产生的定量和定性变化。这意味着自然暴露于DEP可能导致呼吸道变态反应性疾病的表达增加。
Diesel exhaust particles (DEP) have been implicated in the increased incidence of allergic airway disorders. We investigated the effects of DEP on localized immunoglobulin production by performing nasal challenges with varying doses of DEP and analyzing the local immune response in nasal lavages obtained before and after. A significant rise in nasal IgE but not IgG, IgA, IgM, or albumin was observed in subjects 4 d after challenge with 0.30 mg DEP, equivalent to exposure on an average Los Angeles day. Direct evidence for DEP-enhanced local production of IgE was that challenge increased the number of IgE-secreting cells in lavage fluid from < 1 in 2,000,000 to > 1 in 100,000 but did not alter the number of IgA-secreting cells. There was a concomitant increase in epsilon mRNA production in the lavage cells. Additionally, DEP altered the relative amounts of five different epsilon mRNAs generated by alternative splicing, mRNAs that code for different IgE proteins. These results show that DEP exposure in vivo causes both quantitative and qualitative changes in local IgE production. The implication is that natural exposure to DEP may result in increased expression of respiratory allergic disease.