Collection of isolated cells for studying mitochondrial DNA mutations within individual cells.
Collection of isolated cells for studying mitochondrial DNA mutations within individual cells.
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收集分离的细胞，用于研究单个细胞内的线粒体 DNA 突变。
DOI：
10.1007/978-1-59745-521-3_20
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发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Khrapko,Konstantin
中科院分区：
文献类型：
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作者：
Kraytsberg,Yevgenya;Bodyak,Natalya;Myerow,Susan;Nicholas,Alexander;Ebralidze,Konstantin;Khrapko,Konstantin
线粒体基因组完整性是线粒体体细胞遗传学研究的重要课题。定量方法的发展是必不可少的体细胞线粒体遗传学的定量研究，需要表征异质性和突变过程，以及它们对表型发育的影响。本章概述了收集适合于单分子PCR分析mtDNA突变的单个细胞的方法。此外，我们描述了在这些细胞中的呼吸复合物II和IV的协议。总之，在单细胞内识别呼吸缺陷和mtDNA突变提供了评估这些事件在疾病和衰老中的重要性的有力手段。
Mitochondrial genome integrity is an important issue in somatic mitochondrial genetics. Development of quantitative methods is indispensable to somatic mitochondrial genetics as quantitative studies are required to characterize heteroplasmy and mutation processes, as well as their effects on phenotypic developments. This chapter outlines the methods for collecting individual cells appropriate for analysis of mtDNA mutations by single-molecule PCR. In addition, we describe the protocols for respiratory complexes II and IV in these cells. Together, the identification of respiratory deficiency and mtDNA mutations within single cells provides a powerful means of evaluating the importance of these events in disease and aging.