NERVE GROWTH-FACTOR INDUCES TRKA MESSENGER-RNA EXPRESSION IN CULTURED BASAL FOREBRAIN CHOLINERGIC NEURONS FROM 17-DAY FETAL RATS
NERVE GROWTH-FACTOR INDUCES TRKA MESSENGER-RNA EXPRESSION IN CULTURED BASAL FOREBRAIN CHOLINERGIC NEURONS FROM 17-DAY FETAL RATS
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DOI：
10.1016/0304-3940(94)90353-0
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发表时间：
1994-03-14
影响因子：
2.5
通讯作者：
HATANAKA, H
中科院分区：
文献类型：
--
作者：
KOJIMA, M;IKEUCHI, T;HATANAKA, H
我们首先研究了基底前脑组织的高亲和力神经生长因子受体（trkA）基因表达的发育变化。我们的逆转录聚合酶链反应（RT-PCR）分析表明，trkA mRNA存在于这些组织的出生后的大鼠，但不是在那些胎鼠。然后，我们确定的影响，神经生长因子对trkA基因表达的无血清培养的基底前脑神经元从17天的胎鼠。发现添加NGF后36 h可诱导trkA mRNA的表达，而不添加NGF时未检测到trkA mRNA的表达。
We first examined the basal forebrain tissues for developmental changes in expression of the high-affinity NGF receptor (trkA) gene. Our reverse transcriptase polymerase chain reaction (RT-PCR) analysis showed that trkA mRNA is present in those tissues of postnatal rats, but not in those of fetal rats. Then we determined the effect of NGF on trkA gene expression in serum-free cultures of basal forebrain neurons from 17-day fetal rats. NGF was found to induce trkA mRNA 36 h after the addition of NGF, while no trkA mRNA was detected in the absence of NGF.