Effect of curcumin on bone tissue in the diabetic rat: repair of peri-implant and critical-sized defects
Effect of curcumin on bone tissue in the diabetic rat: repair of peri-implant and critical-sized defects
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DOI：
10.1016/j.ijom.2018.04.018
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发表时间：
2018-11-01
影响因子：
2.4
通讯作者：
Ribeiro, F. V.
中科院分区：
文献类型：
--
作者：
Cirano, F. R.;Pimentel, S. P.;Ribeiro, F. V.
本研究确定姜黄素对糖尿病（DM）动物骨愈合的影响。将100只大鼠分为5组：DM + placc、DM + CURC、DM + INS、DM + CURC + INS和非DM组（CURC、姜黄素；placc、安慰剂；INS、胰岛素）。造成严重的颅骨缺损并将钛植入胫骨。对颅骨缺损进行组织学分析，并采用PCR方法定量检测BMP-2、OPN、OPG、RANKL、Runx2、Osx、β -catenin、Lrp-5、Dkk1 mRNA水平。取出植入物进行扭矩评估，收集植入物周围组织进行相同骨相关标记物的mRNA定量分析，并对胫骨进行微计算机断层扫描。与DM + placc组相比，DM + CURC + INS组和非DM组颅骨闭合程度更高（P < 0.05）。与DM + placc组相比，DM + CURC组、DM + CURC + INS组和非DM组种植体固位增加（P < 0.05）。与DM + placc相比，CURC改善了骨体积，增加了骨与种植体的接触（P < 0.05）。在颅骨样本中，与DM + placc相比，CURC有利于调节RANKL/OPG和Dkk1，并改善β -连环蛋白水平（P < 0.05）。与DM + placc相比，CURC可下调种植周样品的Dkk1和RANKL/OPG，上调BMP-2 （P < 0.05）。CURC逆转DM对骨愈合的有害影响，有助于调节骨相关标志物。
This study determined the effect of curcumin on bone healing in animals with diabetes mellitus (DM). One hundred rats were divided into five groups: DM + PLAC, DM + CURC, DM + INS, DM + CURC + INS, and non-DM (CURC, curcumin; PLAC, placebo; INS, insulin). Critical calvarial defects were created and titanium implants were inserted into the tibiae. Calvarial defects were analyzed histometrically, and BMP-2, OPN, OPG, RANKL, Runx2, Osx, beta-catenin, Lrp-5, and Dkk1 mRNA levels were quantified by PCR. The implants were removed for a torque evaluation, the peri-implant tissue was collected for mRNA quantification of the same bone-related markers, and the tibiae were submitted to micro-computed tomography. The DM + CURC + INS and non-DM groups exhibited greater closure of the calvaria when compared to the DM + PLAC group (P < 0.05). Increased retention of implants was observed in the DM + CURC, DM + CURC + INS, and non-DM groups when compared to the DM + PLAC group (P < 0.05). CURC improved bone volume and increased bone-implant contact when compared to DM + PLAC (P < 0.05). In calvarial samples, CURC favourably modulated RANKL/OPG and Dkk1 and improved beta-catenin levels when compared to DM + PLAC (P < 0.05). In peri-implant samples, Dkk1 and RANKL/OPG were down-regulated and BMP-2 up-regulated by CURC when compared to DM + PLAC (P < 0.05). CURC reverses the harmful effects of DM in bone healing, contributing to the modulation of bone-related markers.