Brazilian Journal of Chemical Engineering HYDROLYTIC ENZYMES AS COADJUVANTS IN THE ANAEROBIC TREATMENT OF DAIRY WASTEWATERS
Brazilian Journal of Chemical Engineering HYDROLYTIC ENZYMES AS COADJUVANTS IN THE ANAEROBIC TREATMENT OF DAIRY WASTEWATERS
复制标题
巴西化学工程杂志 水解酶作为乳制品废水厌氧处理中的助剂
DOI：
--
复制
发表时间：
2002
期刊：
影响因子：
--
通讯作者：
D. Freire
中科院分区：
文献类型：
--
作者：
M. M. C. Leal;M. Cammarota;D. Freire
以巴巴苏饼为底物，通过固态发酵获得了一种由限制青霉产生的具有高水平脂肪酶活性（17.2 U.g）的酶提取物。将酶提取物用于水解具有高脂肪含量（180、450、900和1，200 mg.L）的乳品废水。对不同的水解条件进行了试验，确定应在35 ℃的温度下进行，不搅拌，使用10%v/v酶提取物，水解时间为12小时。然后将粗废水和水解废水进行厌氧生物处理。据观察，对于酶预处理的流出物，厌氧处理的效率有显着提高。对于所测试的最高脂肪含量（1，200 mg.L），粗废水和水解废水的去除率分别达到19%和80%。此外，从水解的流出物（1.87公斤COD·m·d）的COD去除率增加了十倍，观察到相对于粗流出物（0.18公斤COD·m·d）。在这项研究中获得的结果说明了使用混合处理（酶-生物）具有高脂肪含量的废水的可行性。
An enzymatic extract produced by Penicillium restrictum having a high level of lipase activity (17.2 U.g) was obtained by solid-state fermentation using babassu cake as substrate. The enzymatic extract was used in the hydrolysis of a dairy wastewater with high fat contents (180, 450, 900 and 1,200 mg.L). Different hydrolysis conditions were tested, and it was determined that it should be carried out at a temperature of 35oC, without agitation, with 10% v/v enzymatic extract and a hydrolysis time of 12 hours. Both crude and hydrolysed effluents were then submitted to an anaerobic biological treatment. It was observed that for the enzymatically pretreated effluent there was a significant improvement in the efficiency of the anaerobic treatment. For the highest fat content tested (1,200 mg.L), removal efficiencies of 19 and 80% were attained for crude and hydrolysed effluents, respectively. In addition, a tenfold increase in the removal rate of COD from the hydrolysed effluent (1.87 kg COD.m.d) was observed in relation to the crude effluent (0.18 kg COD.m.d). The results obtained in this study illustrate the viability of using a hybrid treatment (enzymatic-biological) for wastewaters having high fat contents.