Omega-3 fatty acid supplementation and reduction of traumatic axonal injury in a rodent head injury model Laboratory investigation
Omega-3 fatty acid supplementation and reduction of traumatic axonal injury in a rodent head injury model Laboratory investigation
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DOI：
10.3171/2010.5.jns08914
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发表时间：
2011-01-01
影响因子：
4.1
通讯作者：
Sears, Barry
中科院分区：
文献类型：
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作者：
Mills, James D.;Bailes, Julian E.;Sears, Barry
目的。创伤性脑损伤仍然是西方世界 45 岁以下人群最常见的死亡原因。最近的动物研究证据表明，补充 omega-3 脂肪酸 (O3FA)（特别是二十碳五烯酸 [EPA] 和二十二碳六烯酸 [DHA]）可改善局灶性神经损伤后的功能结果。本研究的目的是确定大鼠弥漫性轴突损伤后补充 O3FA 的益处。方法。使用四十只成年雄性斯普拉格-道利大鼠。三组 10 只大鼠遭受冲击加速损伤，其余组接受假损伤程序（手术，但没有冲击损伤）。其中两组受伤组补充了 10 或 40 mg/kg/天的 O3FA；第三个受伤组作为未补充的对照组。假受伤的大鼠同样没有接受 O3FA 补充。血清脂肪酸水平是通过损伤前和 30 天补充剂结束时分离的血浆磷脂测定的。处死动物后，对脑干白质束进行免疫组织化学分析，以评估β-淀粉样前体蛋白（APP）的存在，这是轴突损伤的标志物。轴突损伤机制的免疫组织化学分析，包括细胞凋亡标记物 caspase-3 的分析； RMO-14，神经丝压实标记物；和细胞色素c（线粒体损伤的标记物）进行了检测。结果。膳食补充富含 EPA 和 DHA 的鱼油浓缩物 30 天后，O3FA 血清水平显着增加：10 mg/kg/天组比初始水平高 11.6% +/- 4.9%，40 mg/kg/天组比初始水平高 30.7% +/- 3.6%。免疫组织化学分析显示显着 (p
Object. Traumatic brain injury remains the most common cause of death in persons under 45 years of age in the Western world. Recent evidence from animal studies suggests that supplementation with omega-3 fatty acid (O3FA) (particularly eicosapentaenoic acid [EPA] and docosahexaenoic acid [DHA]) improves functional outcomes following focal neural injury. The purpose of this study is to determine the benefits of O3FA supplementation following diffuse axonal injury in rats.Methods. Forty adult male Sprague-Dawley rats were used. Three groups of 10 rats were subjected to an impact acceleration injury and the remaining group underwent a sham-injury procedure (surgery, but no impact injury). Two of the groups subjected to the injury were supplemented with 10 or 40 mg/kg/day of O3FA; the third injured group served as an unsupplemented control group. The sham-injured rats likewise received no O3FA supplementation. Serum fatty acid levels were determined from the isolated plasma phospholipids prior to the injury and at the end of the 30 days of supplementation. After the animals had been killed, immunohistochemical analysis of brainstem white matter tracts was performed to assess the presence of beta-amyloid precursor protein (APP), a marker of axonal injury. Immunohistochemical analyses of axonal injury mechanisms including analysis for caspase-3, a marker of apoptosis; RMO-14, a marker of neurofilament compaction; and cytochrome c, a marker of mitochondrial injury- were performed.Results. Dietary supplementation with a fish oil concentrate rich in EPA and DHA for 30 days resulted in significant increases in O3FA serum levels: 11.6% +/- 4.9% over initial levels in the 10 mg/kg/day group and 30.7% +/- 3.6% in the 40 mg/kg/day group. Immunohistochemical analysis revealed significantly (p