The human osteoclast precursor circulates in the monocyte fraction
The human osteoclast precursor circulates in the monocyte fraction
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DOI：
10.1210/en.137.9.4058
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发表时间：
1996-09-01
期刊：
影响因子：
4.8
通讯作者：
Athanasou, NA
中科院分区：
文献类型：
--
作者：
Fujikawa, Y;Quinn, JMW;Athanasou, NA
已知破骨细胞由造血来源的循环单核前体细胞融合形成。这些循环细胞的确切性质，特别是它们与单核细胞的关系尚不清楚。我们已经开发了一种人破骨细胞形成的体外系统，其中人单核细胞[CD 14、CD 11 a、CD 11b和HLA-DR阳性，以及抗酒石酸酸性磷酸酶（TRAP）、降钙素受体（CTR），玻连蛋白受体（VNR）阴性]，并与UMR 106大鼠成骨细胞共培养长达21天。样细胞或ST 2小鼠前脂肪细胞骨髓基质细胞在1 α，25二羟维生素D-3（1，25（OH）（2）D-3）和巨噬细胞集落刺激因子（M-CSF）存在下培养。在这些共培养物中形成了大量TRAP、VNR和CTR阳性多核细胞，能够进行广泛的腔隙性骨吸收;发生这种情况的绝对要求是与上述骨基质细胞、1，25（OH）（2）D-3和M-CSF接触。这些结果表明，人单核破骨细胞前体在单核细胞部分中循环，并表现出单核细胞表型，在分化成成熟功能性破骨细胞的过程中获得破骨细胞表型特征。
The osteoclast is known to be formed by fusion of circulating mononuclear precursor cells of haematopoietic origin. The precise nature of these circulating cells and, in particular, their relation to monocytes is unknown. We have developed an in vitro system of human osteoclast formation whereby human monocytes [CD14, CD11a, CD11b and HLA-DR positive, and tartrate resistant acid phosphatase(TRAP), calcitonin receptor (CTR), vitronectin receptor (VNR) negative] were isolated and cocultured for up to 21 days with UMR106 rat osteoblast-like cells or ST2 mouse preadipocytic bone marrow stromal cells in the presence of 1 alpha,25 dihydroxyvitamin D-3 (1,25(OH)(2)D-3) and macrophage colony stimulating factor (M-CSF). Numerous TRAP, VNR and CTR positive multinucleated cells, capable of extensive lacunar bone resorption, formed in these cocultures; the absolute requirements for this to occur were contact with the above bone stromal cells, 1,25(OH)(2)D-3, and M-CSF. These results show that the human mononuclear osteoclast precursor circulates in the monocyte fraction and exhibits a monocyte phenotype, acquiring osteoclast phenotypic features in the process of differentiation into mature functional osteoclasts.