Rapid effects of plant species diversity and identity on soil microbial communities in experimental grassland ecosystems
Rapid effects of plant species diversity and identity on soil microbial communities in experimental grassland ecosystems
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DOI：
10.1016/j.soilbio.2006.02.009
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发表时间：
2006-08-01
影响因子：
9.7
通讯作者：
Leadley, Paul
中科院分区：
文献类型：
--
作者：
Loranger-Merciris, Gladys;Barthes, Laure;Leadley, Paul
植物群落结构的变化，包括植物多样性的丧失，可能会影响土壤微生物群落。为了验证这一假设，在种植2、3或4种植物的单一栽培或混合栽培的草地上，通过实验改变了植物多样性和组成。我们测试了单一栽培和混合栽培以及植物种类组成对可培养土壤细菌活性、所用底物数量和分解代谢多样性、微生物量N、微生物呼吸和根生物量的影响。这些特性都是在实验播种10个月后测量的。使用Biolog GN和GP微孔板测量了不同植物群落中土壤细菌的活性、使用的底物数量和分解代谢多样性，这是基于氧化还原测试的土壤可培养细菌使用各种有机底物的能力。微生物量N、微生物呼吸和根生物量对植物多样性不敏感。随着植物多样性的下降，可培养土壤微生物活性、所用底物和多样性均下降。它们的活性、底物使用量和多样性在含有3种和4种植物的小区中显著高于单一栽培和含有2种植物的小区。植物种类组成也有影响。四种植物群落的可培养土壤微生物活性和多样性高于任何单一植物群落，这表明植物多样性的影响不能用某一特定植物物种的存在来解释。我们的结果表明，草原生态系统中植物多样性和组成的变化导致了细菌活性和多样性的快速响应。(C)2006爱思唯尔有限公司。保留所有权利。
Changes in plant community structure, including the loss of plant diversity may affect soil microbial communities. To test this hypothesis, plant diversity and composition were experimentally varied in grassland plots cultivated with monocultures or mixtures of 2, 3 or 4 species. We tested the effects of monocultures versus mixtures and of plant species composition on culturable soil bacterial activity, number of substrates used and catabolic diversity, microbial biomass N, microbial respiration, and root biomass. These properties were all measured 10 months after seeding the experiment. Soil bacterial activity, number of substrates used and catabolic diversity were measured in the different plant communities using BIOLOG GN and GP microplates, which are redox-based tests measuring capacity of soil culturable bacteria to use a variety of organic substrates. Microbial biomass N, microbial respiration, and root biomass were insensitive to plant diversity. Culturable soil microbial activity, substrates used and diversity declined with declining plant diversity. Their activity, number of substrates used and diversity were significantly higher in plots with 3 and 4 plant species than in monocultures and in plots with 2 species. There was also an effect of plant species composition. Culturable soil microbial activity and diversity was higher in the four-species plant community than in any of the plant monocultures suggesting that the effect of plant diversity could not be explained by the presence of a particular plant species. Our results showed that changes in plant diversity and composition in grassland ecosystems lead to a rapid response of bacterial activity and diversity. (c) 2006 Elsevier Ltd. All rights reserved.