Quantitative neuroanatomy for connectomics in Drosophila
Quantitative neuroanatomy for connectomics in Drosophila
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DOI：
10.7554/elife.12059
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发表时间：
2016-03-18
期刊：
影响因子：
7.7
通讯作者：
Cardona, Albert
中科院分区：
文献类型：
--
作者：
Schneider-Mizell, Casey M.;Gerhard, Stephan;Cardona, Albert
使用电子显微镜绘制神经元回路需要费力的校对或多个独立重建的协调。在这里，我们描述了新的方法，应用定量乔木和网络的背景，反复校对和重建电路，并创建解剖丰富的布线图。我们测量了新的和现有的果蝇感觉运动（幼虫）和视觉（成人）系统的重建连接的形态学基础。突触输入优先位于许多小的，无微管的“树枝”，分支了一个单一的微管含有“骨干”。遗漏的个别树枝占96%的错误。然而，高度连接的神经元的突触分布在多个树枝上。因此，一个强大的连接到详细的树枝解剖的鲁棒性与重建误差的鲁棒性。通过将迭代重建与多次重建的共识进行比较，我们表明我们的方法通过发现和应用细胞神经解剖学和突触连接之间的关系克服了冗余工作的需要。
Neuronal circuit mapping using electron microscopy demands laborious proofreading or reconciliation of multiple independent reconstructions. Here, we describe new methods to apply quantitative arbor and network context to iteratively proofread and reconstruct circuits and create anatomically enriched wiring diagrams. We measured the morphological underpinnings of connectivity in new and existing reconstructions of Drosophila sensorimotor (larva) and visual (adult) systems. Synaptic inputs were preferentially located on numerous small, microtubule-free 'twigs' which branch off a single microtubule-containing 'backbone'. Omission of individual twigs accounted for 96% of errors. However, the synapses of highly connected neurons were distributed across multiple twigs. Thus, the robustness of a strong connection to detailed twig anatomy was associated with robustness to reconstruction error. By comparing iterative reconstruction to the consensus of multiple reconstructions, we show that our method overcomes the need for redundant effort through the discovery and application of relationships between cellular neuroanatomy and synaptic connectivity.