Observation of Stomatal Movements of Intact Plants Using an Image Instrumentation System with a Light Microscope
Observation of Stomatal Movements of Intact Plants Using an Image Instrumentation System with a Light Microscope
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使用光学显微镜图像仪器系统观察完整植物的气孔运动
DOI：
10.1093/pcp/24.2.281
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发表时间：
1983
影响因子：
4.9
通讯作者：
I. Aiga
中科院分区：
文献类型：
--
作者：
K. Omasa;Y. Hashimoto;I. Aiga
为了观察完整植物在生长条件下对环境变化的气孔反应，我们开发了带有光学显微镜的远程控制图像仪器系统。该系统由以下部分组成：(1) 具有宽工作距离 (13 mm)、高放大倍率（电视监视器上放大倍率约 1,600 倍）的光学显微镜； (2) 一个可移动的显微镜载物台，其设计允许经过调节的空气沿着固定叶片的两侧通过，并从上方和下方进行照明； (3)高灵敏度SIT相机(S20型光谱响应)和高分辨率、畸变小的单色电视监视器，用于在单独的房间观察显微镜图像(可以用约0.1 mW/cm2以上的单一反射或透射光观察气孔)； (4) 远程控制器可调节相机灵敏度、显微镜焦距以及从独立观察室移动显微镜图像的视野。该系统解决了普通光学显微镜在观察完整生长植物的气孔运动时遇到的问题。由于操作简单、快速，该系统还可用于观察许多完整的气孔。此外，使用该系统可以准确确定气孔孔径以及角质层蒸腾与气孔蒸腾的比率。
In order to observe the stomatal response of intact plants to environmental changes under growing conditions, we developed a remote-control image instrumentation system with a light microscope. This system is composed of: (1) a light microscope with a wide working distance (13 mm) at high magnification (ca. 1,600-fold magnification on a TV monitor); (2) a movable microscope stage designed to permit the passage of conditioned air along both sides of a fixed leaf and for illuminating from above and below; (3) a high-sensitivity SIT camera (S20 type spectral response) and a monochromatic TV monitor with high resolution and small distortion, used to observe the microscope image in a separate room (stomata can be observed with single reflected or transmitted light above ca. 0.1 mW/cm2); (4) remote controllers to adjust camera sensitivity, microscope focus and movement of the visual field of the microscope image from the separated observation room. This system solved the problems encountered with an ordinary light microscope in observing stomatal movement of intact growing plants. This system also can be used to observe many intact stomata because of its easy and rapid operation. Furthermore, the stomatal aperture and the ratio of transpiration from the cuticle to that from the stomata can be accurately determined using this system.