Fission yeast cut1 and cut2 are essential for sister chromatid separation, concentrate along the metaphase spindle and form large complexes
Fission yeast cut1 and cut2 are essential for sister chromatid separation, concentrate along the metaphase spindle and form large complexes
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DOI：
10.1002/j.1460-2075.1996.tb01052.x
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发表时间：
1996-12-02
期刊：
影响因子：
11.4
通讯作者：
Yanagida, M
中科院分区：
文献类型：
--
作者：
Funabiki, H;Kumada, K;Yanagida, M
裂殖酵母粟酒裂殖酵母温度敏感（ts）cut 1突变体不能在后期分离姐妹染色单体，但细胞继续分裂，导致未分裂的细胞核一分为二（切割表型）。如果胞质分裂被阻断，复制继续进行，形成一个巨大的细胞核与多倍体染色体，我们在这里表明，ts cut 2 -364的表型是高度相似的cut 1和cut 1（+）和cut 2（+）的基因产物的功能是密切相关的。cut 1（+）和cut 2（+）基因对生存力至关重要，并在遗传上相互作用。Cut 1蛋白在中期沿着短纺锤体沿着集中，Cut 2也是如此。Cut 1（类似于200 kDa）和Cut 2（42 kDa）缔合，如通过免疫沉淀所示，并且在蔗糖梯度离心中共沉淀为大复合物（30和40 S）。它们在细胞周期中的行为是显著不同的，然而：Cut 2在后期被用于破坏细胞周期蛋白B的相同蛋白水解机制降解，而Cut 1存在于整个细胞周期。Cut 1-Cut 2复合体的基本功能是确保姐妹染色单体分离，这可能是由Cut 2蛋白水解调节。Cut 1的C-末端区域在进化上是保守的，并且与芽殖酵母Esp 1、丝状真菌BimB和人类蛋白质的C-末端区域相似。
Fission yeast Schizosaccharomyces pombe temperature-sensitive (ts) cut1 mutants fail to separate sister chromatids in anaphase but the cells continue to divide, leading to bisection of the undivided nucleus (the cut phenotype). If cytokinesis is blocked, replication continues, forming a giant nucleus with polyploid chromosomes, We show here that the phenotype of ts cut2-364 is highly similar to that of cut1 and that the functions of the gene products of cut1(+) and cut2(+) are closely interrelated. The cut1(+) and cut2(+) genes are essential for viability and interact genetically. Cut1 protein concentrates along the short spindle in metaphase as does Cut2. Cut1 (similar to 200 kDa) and Cut2 (42 kDa) associate, as shown by immunoprecipitation, and cosediment as large complexes (30 and 40S) in sucrose gradient centrifugation, Their behavior in the cell cycle is strikingly different, however: Cut2 is degraded in anaphase by the same proteolytic machinery used for the destruction of cyclin B, whereas Cut1 exists throughout the cell cycle. The essential function of the Cut1-Cut2 complex which ensures sister chromatid separation may be regulated by Cut2 proteolysis. The C-terminal region of Cut1 is evolutionarily conserved and similar to that of budding yeast Esp1, filamentous fungi BimB and a human protein.