G-banding and molecular cytogenetic analyses of marginal zone lymphoma
G-banding and molecular cytogenetic analyses of marginal zone lymphoma
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DOI：
10.1111/j.1365-2141.2005.05706.x
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发表时间：
2005-09-01
影响因子：
6.5
通讯作者：
Heim, S
中科院分区：
文献类型：
--
作者：
Aamot, HV;Micci, F;Heim, S
我们通过各种全基因组细胞遗传学技术分析了22例边缘带淋巴瘤（MZL）患者的获得性染色体畸变，如g带、多色荧光原位杂交（M-FISH）、跨物种彩色带（RxFISH）和比较基因组杂交（CGH），以及带有位点特异性探针的FISH。重排最多的3号染色体异常患者（n = 11）的中位生存期短于3号染色体正常患者（n = 11, 74个月对219个月，P < 0.03）。淋巴结型MZL患者中有4 / 5存在3号染色体异常，且淋巴结型MZL患者的中位生存期短于其他形态学亚组MZL患者（P < 0.003）。CGH分析显示，各2- 4例（20-40%）仅获得染色体物质，即染色体区域3p12-25、3q12-21、3q23-28、12q13-15、12q22-24、19p13和19q13。在两个MZL中，新的不平衡易位der(13)t(3;13)（q24;p11）被检测到是唯一的核型重排，表明3q24-qter的获得可能是这些淋巴瘤发病机制中的一个重要事件。另外两个病例除了其他异常外，还显示t(4;14)（p13;q32）。这两种淋巴瘤都涉及到位于14q32的IGH基因，其中一种也涉及到位于4p13的RHOH/TTF基因，该基因编码RHO蛋白亚家族的新成员。
We analysed the acquired chromosomal aberrations of 22 marginal zone lymphoma (MZL) patients by various genome-wide cytogenetic techniques, such as G-banding, multicolour fluorescence in situ hybridisation (M-FISH), cross-species colour banding (RxFISH), and comparative genomic hybridisation (CGH), as well as FISH with locus-specific probes. Patients with an abnormal chromosome 3 (n = 11), the most frequently rearranged chromosome, showed a shorter median survival than patients with a normal chromosome 3 (n = 11, 74 months vs. 219 months, P < 0.03). Four of five patients with nodal MZL had chromosome 3 abnormalities and patients with nodal MZL had a shorter median survival than patients in the other morphological subgroups of MZL (P < 0.003). CGH analysis showed only gains of chromosome material, namely of chromosome regions 3p12-25, 3q12-21, 3q23-28, 12q13-15, 12q22-24, 19p13 and 19q13 in two to four cases each (20-40%). In two MZL, the novel unbalanced translocation der(13)t(3;13)(q24;p11) was detected as the sole karyotypic rearrangement, indicating that gain of 3q24-qter could be an important event in the pathogenesis of these lymphomas. Another two cases showed, in addition to other abnormalities, a t(4;14)(p13;q32). Both these lymphomas had involvement of the IGH gene at 14q32, and one of them also of the RHOH/TTF gene at 4p13, which encodes a new member of the RHO protein subfamily.