Hatchery decontamination of Sargassum muticum juveniles and adults using a combination of sodium hypochlorite and potassium iodide
Hatchery decontamination of Sargassum muticum juveniles and adults using a combination of sodium hypochlorite and potassium iodide
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DOI：
10.1007/s10811-015-0672-8
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发表时间：
2016-04-01
影响因子：
3.3
通讯作者：
Hughes, Adam D.
中科院分区：
文献类型：
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作者：
Kerrison, Philip D.;Hau Nhu Le;Hughes, Adam D.
马尾藻属的褐藻类大型藻类，包括鼠尾马尾藻，正在作为栽培作物进行研究。过度生长或放牧会降低作物的价值和生产力。这可能发生在孵化场和海上生长期间，尽管幼年阶段特别脆弱。可以使用去污协议来防止这种情况。筛选程序后，选择次氯酸钠（NaClO）和碘化钾（KI）进行进一步研究。与S.突变合子为1%KI、0.75%NaClO或0.5%KI和0.38%NaClO的组合。暴露于这些处理3分钟能够在20- 80%的时间内从小枝中消除原生动物。NaClO导致光系统II的操作效率（F-q（'）/F-m（'））大幅降低，特别是在17天后仍未完全恢复的青少年中（74 +/-17%）。这也使幼鱼密度减半，造成负增长。1%的KI使成人的F-q（'）/F-m（'）降低29 +/-11%，第10天恢复。在幼鱼中，生长下降65%，F-q（'）/F-m（'）下降61 +/-11%，17天后恢复。0.5% KI和0.38% NaClO的联合处理具有温和得多的效果：使成虫和幼虫的F-q（'）/F-m（'）分别降低6 +/- 3和34 +/-9%，到第10天完全恢复。在第0-10天之间也观察到幼体生长减少（32%）;然而，到第20天，大小与对照组没有差异。因此，这种组合处理适合于青少年和成年人的去污。粘液组织
Phaeophyte macroalgae of the genus Sargassum, including Sargassum muticum, are under investigation as a cultivation crop. Overgrowth or grazing reduces the value and productivity of the crop. This can occur both in the hatchery and during growth at sea, although juvenile stages are especially vulnerable. A decontamination protocol could be used to prevent this. Following a screening procedure, sodium hypochlorite (NaClO) and potassium iodide (KI) were selected for further study. The lethal concentrations for protozoans associated with S. muticum zygotes were 1 % KI, 0.75 % NaClO or a combination of 0.5 % KI and 0.38 % NaClO. A 3-min exposure to these treatments was able to eliminate protozoa from branchlets 20-80 % of the time. NaClO caused large reductions in the operating efficiency of photosystem II (F-q (')/F-m (')), particularly in juveniles (74 +/- 17 %), which had still not fully recovered after 17 days. This also halved juvenile density and caused negative growth. One percent KI reduced F-q (')/F-m (') in adults by 29 +/- 11 %, with recovery by day 10. In juveniles, growth was reduced by 65 % and F-q (')/F-m (') by 61 +/- 11 % with recovery after 17 days. A combined treatment of 0.5 % KI and 0.38 % NaClO had far milder effects: reducing adult and juveniles F-q (')/F-m (') by 6 +/- 3 and 34 +/- 9 % respectively, with full recovery by day 10. A reduction in juvenile growth was also seen between day 0-10 (32 %); however, size was no different to the control by day 20. This combined treatment is therefore suitable for decontamination of both juveniles and adult S. muticum tissue.