Identification of NCAM that interacts with the PHE-CoV spike protein.
Identification of NCAM that interacts with the PHE-CoV spike protein.
复制标题
鉴定与 PHE-CoV 刺突蛋白相互作用的 NCAM
DOI：
10.1186/1743-422x-7-254
复制
发表时间：
2010-09-24
期刊：
影响因子：
4.8
通讯作者：
Gao F
中科院分区：
文献类型：
--
作者：
Gao W;He W;Zhao K;Lu H;Ren W;Du C;Chen K;Lan Y;Song D;Gao F
背景冠状病毒的刺突蛋白与细胞分子结合以介导其靶细胞的感染。病毒感染所需的细胞蛋白的表征对于了解病毒生命周期至关重要，并可能为抗病毒治疗提供细胞靶标。结果我们确定神经细胞粘附分子（NCAM）是 PHE-CoV S 蛋白的新型相互作用伙伴。构建了 N2a 细胞的 T7 噬菌体展示 cDNA 文库，并用可溶性 PHE-CoV S 糖蛋白筛选该文库。我们使用免疫共沉淀测定表明只有 NCAM 是刺突蛋白的结合伴侣。我们发现可溶形式的抗 NCAM 抗体阻断了 PHE-CoV 与 N2a 细胞的结合。此外，针对 NCAM 的双链 siRNA 抑制了 PHE-CoV 感染。结论 NCAM 和刺突蛋白之间发现了一种新的相互作用，这种关联在 PHE-CoV 感染期间至关重要。
BackgroundThe spike proteins of coronaviruses associate with cellular molecules to mediate infection of their target cells. The characterization of cellular proteins required for virus infection is essential for understanding viral life cycles and may provide cellular targets for antiviral therapies.ResultsWe identified Neural Cell Adhesion Molecule (NCAM) as a novel interacting partner of the PHE-CoV S protein. A T7 phage display cDNA library from N2a cells was constructed, and the library was screened with the soluble PHE-CoV S glycoproteins. We used a coimmunoprecipitation assay to show that only the NCAM was a binding partner of spike protein. We found that a soluble form of anti-NCAM antibody blocked association of the PHE-CoV with N2a cells. Furthermore, double-stranded siRNA targeted against NCAM inhibited PHE-CoV infection.ConclusionsA novel interaction was identified between NCAM and spike protein and this association is critical during PHE-CoV infection.