Involvement of the C‐terminal domain in cell surface localization and G‐protein coupling of mGluR6
Involvement of the C‐terminal domain in cell surface localization and G‐protein coupling of mGluR6
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C 末端结构域参与 mGluR6 的细胞表面定位和 G 蛋白偶联
DOI：
10.1111/jnc.15217
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发表时间：
2020
影响因子：
4.7
通讯作者：
Kaneda Makoto
中科院分区：
文献类型：
--
作者：
Rai Dilip;Akagi Takumi;Shimohata Atsushi;Ishii Toshiyuki;Gangi Mie;Maruyama Takuma;Wada‐Kiyama Yuko;Ogiwara Ikuo;Kaneda Makoto
代谢型谷氨酸受体6（mGluR 6）通过其细胞内C末端结构域（CTD）与支架蛋白和Gβγ亚基相互作用。mGluR 6通路在视网膜处理视觉信号中起关键作用。我们在此使用mGluR 6-CTD突变体，通过免疫细胞化学、表面生物素化测定和电生理方法研究CTD（残基840-871）是否是mGluR 6细胞表面定位和G-蛋白偶联所必需的。我们使用293 T细胞和原代海马神经元作为模型系统。我们检查了C端截短的mGluR 6，发现去除多达858个残基不会影响表面定位或谷氨酸诱导的G蛋白介导的反应，而15个氨基酸缺失（Δ857 - 871）会损害这些功能。然而，21个氨基酸的缺失（Δ851 - 871）恢复了表面定位和谷氨酸依赖性反应，当整个CTD被去除时，这些反应再次减弱。III组mGluRs的序列比对显示CTD中类似ER保留基序的保守氨基酸。这些结果表明，细胞内CTD所需的mGluR 6的细胞表面运输和受体功能，而它可能包含调节元件的细胞内运输和信号。
Metabotropic glutamate receptor 6, mGluR6, interacts with scaffold proteins and Gβγ subunits via its intracellular C‐terminal domain (CTD). The mGluR6 pathway is critically involved in the retinal processing of visual signals. We herein investigated whether the CTD (residues 840–871) was necessary for mGluR6 cell surface localization and G‐protein coupling using mGluR6‐CTD mutants with immunocytochemistry, surface biotinylation assays, and electrophysiological approaches. We used 293T cells and primary hippocampal neurons as model systems. We examined C‐terminally truncated mGluR6 and showed that the removal of up to residue 858 did not affect surface localization or glutamate‐induced G‐protein‐mediated responses, whereas a 15‐amino acid deletion (Δ857‐871) impaired these functions. However, a 21‐amino acid deletion (Δ851‐871) restored surface localization and glutamate‐dependent responses, which were again attenuated when the entire CTD was removed. The sequence alignment of group III mGluRs showed conserved amino acids resembling an ER retention motif in the CTD. These results suggest that the intracellular CTD is required for the cell surface transportation and receptor function of mGluR6, whereas it may contain regulatory elements for intracellular trafficking and signaling.
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DOI：
10.1073/pnas.0611577104
发表时间：
2007-03-06
影响因子：
11.1
作者：
Muto, Takanori;Tsuchiya, Daisuke;Jingami, Hisato
通讯作者：
Jingami, Hisato
影响因子：
3.6
作者：
Gassmann, M;Haller, C;Bettler, B
通讯作者：
Bettler, B
DOI：
10.1073/pnas.0501233102
发表时间：
2005-03-29
影响因子：
11.1
作者：
Dryja, TP;McGee, TL;Rajagopalan, AS
通讯作者：
Rajagopalan, AS
影响因子：
6.1
作者：
Singh, Baljinder;Ogiwara, Ikuo;Yamakawa, Kazuhiro
通讯作者：
Yamakawa, Kazuhiro
通过与微管和肌动蛋白流结合进行代谢型谷氨酸受体的主动表面转运
影响因子：
4
作者：
Arnauld Sergé;L. Fourgeaud;A. Hémar;D. Choquet
通讯作者：
D. Choquet