Over-expression of sly-miR156a in tomato results in multiple vegetative and reproductive trait alterations and partial phenocopy of the sft mutant
Over-expression of sly-miR156a in tomato results in multiple vegetative and reproductive trait alterations and partial phenocopy of the sft mutant
复制标题
番茄中 sly-miR156a 的过度表达导致 sft 突变体的多种营养和生殖性状改变以及部分表型
DOI：
10.1016/j.febslet.2010.12.036
复制
发表时间：
2011-01-21
期刊：
影响因子：
3.5
通讯作者：
Ye, Zhibiao
中科院分区：
文献类型：
--
作者：
Zhang, Xiaohui;Zou, Zhe;Ye, Zhibiao
植物microRNAs（miRNAs）是植物发育和繁殖的翻译控制系统的重要组成部分。通过在番茄植物中过表达研究了sly-miR 156的生物学功能。转基因番茄植株表现出显著改变的表型，高度降低，叶片变小但数量增多，果实变小。sly-miR 156过表达植物的花序结构表型模仿sft突变体。通过数据库检索鉴定了sly-miR 156的推定靶标，包括六个鳞状细胞启动子结合蛋白（SBP）盒转录因子基因。然后在35 S-miR 156 a和野生型番茄植物中确定它们的表达模式。这些靶基因，以及番茄FLOWERLOCUS T（FT）直系同源物SFT，在sly-miR 156过表达植物中显著下调。这些研究揭示了miR 156调控的新表型。(C)2010年欧洲生物化学学会联合会。Elsevier B. V.出版，保留所有权利。
Plant microRNAs (miRNAs) are vital components of the translation control system that regulates plant development and reproduction. The biological function of sly-miR156 was investigated by over-expression in tomato plants. Transgenic tomato plants exhibited a drastically altered phenotype, with reduced height, smaller but more numerous leaves, and smaller fruit. The inflorescence structure of sly-miR156 over-expressing plants phenocopied the sft mutant. The putative targets of sly-miR156 were identified by data base search and included six SQUAMOSA PROMOTER BINDING PROTEIN (SBP)-box transcription factor genes. Their expression patterns were then determined in 35S-miR156a and wild type tomato plants. These target genes, as well as the tomato FLOWERING LOCUS T (FT) ortholog SFT, were significantly down-regulated in sly-miR156 over-expressing plants. These studies reveal novel phenotypes regulated by miR156. (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.